荧光染料重叠最小化：尽量选择光谱重叠小的荧光素，如fitc/pe-cy7；选择不同激光激发的荧光素，如fitc/apc，pe/apc。 光谱重叠严重的荧光素同时检测会导致荧光溢漏，需调节补偿。

PE-Cy7 is a near-IR dye used primarily in flow cytometry for low-abundance antigens. We offer PE-Cyanine7 dye conjugated to a variety of antibodies, peptides, and proteins, optimized for cellular labeling and detection.

今天崔工跟大家简单的介绍下 藻红蛋白Cy7 这款复合染料（ PE-Cy7)吧。 一、产品简介. PE-Cy7是一种复合 荧光素 。 由PE和Cy7这两种荧光素通过共价结合在一起。 荧光素在受到适当波长的光线照射时，会释放出波长比照射光长的光，这个现象叫做荧光。

PE-Cyanine®7 (PE-Cy7, RPE-Cy7) is a far red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy7. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy7, which emits light that can be captured with a 780/60 nm bandpass filter.

PE-Cyanine7 是一种可以为广泛种类激光（包括 488 nm、 532 nm 和 561 nm）所激发的串联荧光团。 如果暴露于光漂白作用、固定过程和更高温度，染料降解常见。

PE-Cy5是一种复合染料，属于藻红蛋白偶联物，在此类复合染料中，激发能量可从PE传递到Cy5上，最大发射波长为667nm。 由于PE-Cy5与FITC、PE在光谱上重叠范围小，荧光干扰少，因此实验中常将PE-Cy5与FITC、PE搭配使用。

Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor. The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher.

2023年11月23日 · PE-Cy7是流式细胞术中常用的颜色。 其主要吸收峰位于565 nm，发射峰位于780 nm。 提供预活化PE-Cy7，以促进PE-Cy7串联结合抗体和其他蛋白质，如链霉抗生物素蛋白和其他二级试剂。

This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm.

2017年8月28日 · APC-Cy7是由APC 和Cy7组合而成的Tandem荧光素；其标记的抗体适用于配备氦氖激光器发射波长为633nm的多色流式细胞仪，APC-Cy7主要用于四色以上的流式细胞术。

PE-Cy7 复合荧光素是将藻胆蛋白PE 和有机荧光染料Cy7 通过化学偶联制成，具有荧 光强度高，Stokes 位移大的特点。 同时，利用复合荧光素具有PE 荧光强度减弱而Cy7 荧光

PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher.

PE-Cy7 是一种复合荧光素。 由 PE 和 Cy7 这两种荧光素通过共价结合在一起。 荧光素在受到适当波长的光线照射时，会释放出波长比照射光长的光，这个现象叫做荧光。

2023年11月21日 · PE-Cy7是流式细胞术中常用的颜色。 其主要吸收峰位于565 nm，发射峰位于780 nm。 提供预活化PE-Cy7，以促进PE-Cy7串联结合抗体和其他蛋白质，如链霉抗生物素蛋白和其他二级试剂。

Immunofluorescent Staining and Flow Cytometric Analysis: The PE-Cy™7 Mouse Anti-Human IFN-γ antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate IFN-γ producing cells within mixed cell populations.

PE-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at 780 nm. PE-Cy7 Tandem is manufactured by AAT Bioquest

PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher.

PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher.