Pulsed-field gel electrophoresis (PFGE) is a technique used for the separation of large DNA molecules by applying an electric field that periodically changes direction to a gel matrix. [1][2] Unlike standard agarose gel electrophoresis, which can separate DNA fragments of up to 50 kb, PFGE resolves fragments up to 10 Mb. [1] .

Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction.

2022年8月23日 · Pulsed Field Gel Electrophoresis (PFGE) is a technique used for the separation of large deoxyribonucleic acid (DNA) molecules by applying to a gel matrix an electric field that periodically changes direction.

PFGE是 脉冲场电泳 的的英文缩写，脉冲场电泳是利用交变电场使得在DNA分子在 琼脂糖凝胶 中改变方向，按照不同的速度跑出一系列条带，研究者根据条带来进行鉴定与溯源等工作。 不同的DN分子在电场中迁移速度依据分子量的大小，分子越小移动的速度越快，分子量越大移动速度越慢。 脉冲场凝胶电泳可以区分大小在20-1200kb之间的DNA分子。 总体来说，脉冲场凝胶电泳是一种高度区分性的分子分型技术，也是国内外监测程序中的金标准，其通过比较任何两个分析株的 …

2024年4月3日 · Pulsed-field gel electrophoresis (PFGE) is a powerful technique used to separate large DNA molecules based on their size. It involves applying an electric field that periodically changes direction to a gel matrix, allowing for the effective separation of DNA molecules.

Two different strains of Salmonella Newport digested with BlnI. Vibrio cholerae digested with high fidelity NotI enzyme (NEB); not recommended for use with PFGE. If there is indentation, then it is a doublet. (light and dark can be resolved), then it is …

Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for bacteria typing. The method involves enzyme restriction of bacteria DNA, separation of the restricted DNA bands using a pulsed-field electrophoresis chamber, followed by clonal assignment of bacteria based on PFGE banding patterns.

Pulsed Field Gel Electrophoresis, or PFGE, is a technique used for the separation of very large DNA molecules. Under standard electrophoresis conditions, an electric field is applied in one direction on the agarose gel, and DNA molecules larger than 40 kilobases essentially move together through the gel, independently of their size.

Pulsed-field gel electrophoresis (PFGE) is a powerful molecular typing technique by which genomic DNA is isolated from the organism of interest, followed by restriction enzyme analysis.

2010年10月1日 · Remarkably, despite (or perhaps because of) its more than 20-year history as a typing method, PFGE has demonstrated exceptional staying power. The reasons for this endurance as well as the pros and cons of PFGE use are examined in this review. In broad context the history and technology behind PFGE are considered.