Here we report that PKR activation by PACT causes cellular apoptosis in addition to PKR autophosphorylation and translation inhibition. We analyzed the structure-function relationship …

2024年9月18日 · Protein kinase R (PKR) functions in the eukaryotic innate immune system as a first-line defense against viral infections. PKR binds viral dsRNA, leading to …

When bound to an activating RNA and ATP, PKR undergoes autophosphorylation reactions at multiple serine and threonine residues. This autophosphorylation reaction stimulates the eIF2α …

2007年2月1日 · The double-stranded (ds) RNA-activated protein kinase, PKR, has a key role in the innate immunity response to viral infection in higher eukaryotes. PKR contains an N …

2016年6月16日 · PKR是一个551个氨基酸残基组成的蛋白激酶，由两个功能部分组成，一个N末端的 RNA结合结构域和一个C末端的激酶结构域，这两个结构域之间通过一个80个氨基酸残

2017年8月1日 · A few ATP competitive inhibitors of the PKR have been reported in the literature with promising results in vitro and in vivo. The aim of this study was to unravel the structural …

RNA-generated phosphorylated PKR (PKR TAR-P) was made by incubating wild-type full-length human PKR (1 μ m) and HIV TAR RNA in the presence of ATP (1 m m) and MgCl 2 (2 m m) at …

2022年1月20日 · Exon back-splicing-generated circular RNAs, as a group, can suppress double-stranded RNA (dsRNA)-activated protein kinase R (PKR) in cells. We have sought to …

2023年9月1日 · Pyruvate kinase (PK) is a glycolytic enzyme, which catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, resulting in generation of adenosine triphosphate …

Model for PKR activation. PKR exists in an open conformation (i) in a weak monomer-dimer equilibrium. Dimerization is induced by binding to dsRNA (ii) or at high protein concentration …