Pocket Modification of ω-Amine Transaminase AtATA for …
2023年11月1日 · Strengthening of intramolecular interactions and expansion of the angle between the substrate-binding pocket and the pyridoxal 5′-phosphate (PLP)-binding pocket …
ω-转氨酶分子改造研究进展 - Chinese Journal of Biochemistry ...
在近期一项关于荧光假单胞菌Pseudomonas fluorescens来源的S型ω-转氨酶的稳定性改造研究中，Börner等 基于底物诱导酶失活的机理将突变区域限制在辅酶结合区域，对该区域32个位点 …
Reprogramming biocatalytic futile cycles through computational ...
2024年1月2日 · To explore a plausible strategy for creating an AR, we compared two catalytically related enzymes: amino acid racemase (AAR) and ATA, both of which are pyridoxal 5′ …
Characterization of the stability of Vibrio fluvialis JS17 amine ...
2018年9月20日 · In this work, the stability of Vf -ATA was explored. First, the affinity between enzyme and pyridoxal-5'-phosphate (PLP) (KD value of 7.9 μM) was determined. Addition of …
Creation of a robust and R-selective ω-amine ... - ScienceDirect
2020年11月1日 · Compared with the asymmetric hydrogenation of enamines catalyzed by a transition metal, asymmetric transamination catalyzed by ω-amine transaminase (ω-ATA) …
Expanding the Toolbox of R‐Selective Amine Transaminases by ...
2020年11月26日 · Amine transaminases (ATAs) are used to synthesize enantiomerically pure amines, which are building blocks for pharmaceuticals and agrochemicals. R -selective ATAs …
磷酸吡哆醛依赖型酶的研究进展及其应用
This article details the structural features and catalytic mechanisms of typical PLP-dependent enzymes such as ω-transaminase, lysine decarboxylase, threonine aldolase, and L-tyrosine …
Structural insight into the substrate specificity of PLP fold type IV ...
2020年1月27日 · In this review, we aim to analyze the substrate profiles and correlations between the substrate specificity and organization of the active site in transaminases from these …
锐谈 | ACS Catal: D-氨基酸氨基转移酶的定向进化产生（R）-β-转 …
2023年5月16日 · 吡哆醛-5′-磷酸（PLP）依赖性转氨酶（TAs）根据对不同的氨基供体大致分为α-或ω-TA，ω-TAs可进一步分为两个亚组，即β-TAs和胺转氨酶（ATAs）。 ATAs 可以将氨基 …
Insight into the dimer dissociation process of the
2019年11月18日 · Recently published data support the hypothesis of a key role for the cofactor pyridoxal-5′-phosphate (PLP) in the stability of both dimeric and tetrameric (S)-selective …