PTM-Signature Enrichment Analysis (PTM-SEA) is a modified version of ssGSEA to perform site-specific signature analysis by scoring PTMsigDB's bi-directional signature-sets. The input to PTM-SEA is a single site-centric data matrix, m, stored in GCT v1.2 or GCT v1.3 format and PTM signatures database

景杰生物提供PTM-SEA（PTM-Signature Enrichment Analysis）分析服务，通过磷酸化位点识别信号通路的失调，进行特征富集分析，为疾病机制、医疗诊断和药物研发提供重要参考。

Single sample Gene Set Enrichment analysis (ssGSEA) and PTM Enrichment Analysis (PTM-SEA) Resources

We adapted the widely used single sample Gene Set Enrichment Analysis approach to utilize PTMsigDB, enabling PTM S ignature E nrichment A nalysis (PTM-SEA) of quantitative MS data. We used a well-characterized data set of epidermal growth factor (EGF)-perturbed cancer cells to evaluate our approach and demonstrated better representation of ...

分析时，作者重点关注树状图中的顶部分裂，应用了位点特异性通路富集分析—ptm-sea，以及基于底物特异性预测ptm调节因子的激酶库（star方法中的ptm专用工具），两种工具均显示，在第一次分裂的左侧，细胞周期蛋白依赖性激酶（cdk）活性显著富集，p21活化 ...

2021年8月26日 · PTM-SEA 分析发现， CDK1 、 CDK2 、 CDC7 、 CHEK2 和 MELK 等 kinase-substrate signatures 显著上调，为食管癌的靶向治疗提供了候选靶点。此外，与食管癌已知的基因组突变数据整合分析，补充了基因组、转录组研究的不足，有助于从多维度深刻理解食管癌致病机 …

2021年4月16日 · Single sample Gene Set Enrichment analysis (ssGSEA) and PTM Enrichment Analysis (PTM-SEA)

We adapted the widely used single sample Gene Set Enrichment Analysis approach to utilize PTMsigDB, enabling ignature nrichment nalysis (PTM-SEA) of quantitative MS data. We used a well-characterized data set of epidermal growth factor (EGF)-perturbed cancer cells to evaluate our approach and demonstrated better representation of signaling ...

2019年3月1日 · PTM signature enrichment analysis (PTM-SEA) outperformed gene-centric analysis in detection of EGF induced phospho signaling events. PI3K perturbation signatures were readily detected in PI3Ka inhibited human breast cancer cells. PTMsigDB and PTM-SEA can be freely accessed at https://github.com/broadinstitute/ssGSEA2.0.

2024年4月29日 · Here we describe the high-throughput, functional assessment of phosphorylation sites through the development of PTM-centric base editing coupled to phenotypic screens, directed by temporally ...