PAmCherry1 is a photoactivatable red fluorescent protein published in 2009, derived from Discosoma sp.. It is reported to be a rapidly-maturing monomer with high acid sensitivity.

2009年1月25日 · Here we report several irreversibly photoactivatable derivatives of mCherry 17, named PAmCherry proteins, with excellent photoactivation contrast over background, advanced photostability and high...

2009年12月15日 · Photoactivatable fluorescent proteins (PAFPs) are required for super-resolution imaging of live cells. Recently, the first red PAFP, PAmCherry1, was reported, which …

Takara (公司 推出光激活荧光蛋白 PAmCherry 蛋白的动力学,如蛋白移位和降解等。 过光激活荧光蛋白,您还可以实时观察 察到的正是激活时蛋白分子的快照。

Plasmid PAmCherry1-C1-P4C from Dr. Gerry Hammond's lab contains the insert PAmCherry1: L. pneumophila SidC (608-773) and is published in J Cell Biol. 2023 Feb 6;222 (2):e202204099. doi: 10.1083/jcb.202204099. Epub 2022 Nov 23. This plasmid is available through Addgene.

红色荧光蛋白变种mCherry是一种目前被广泛用于生物技术作为示踪剂的红色 荧光染料，包括分子的标记和细胞组分的定位等。 mCherry因为其颜色和单体分子的 光稳定性，细胞毒性低。 比其它荧光蛋白标签更优异，其最大激发光和发射光分别为587nm和610nm。 从结构上看突变体mcherry能够具有如此强的竞争力有2个原因。 1，mcherry中的第163位Lys已经突变成了Gln，这个突变的氨基酸所在的侧链位于发色团苯环下方，在结构中能够清楚的看到163位的Lys与发色 …

最近，报道了第一个红色 PAFP，PAmCherry1，它通过提供红色超分辨率颜色来补充可光激活的 GFP。 PAmCherry1 最初是“暗色”，但在紫外-紫光照射后呈现红色荧光。 为了定义 PAmCherry1 光活化的结构基础，我们分别在 1.50 A 和 1.65 A 下确定了其在暗和红色荧光状态下的晶体结构。 黑暗中发色团的非共面结构 PAmChery1 表明在环化 Met-66 的 Tyr-67 侧链中存在 N-酰基亚胺官能团和单个非氧化 C (alpha)-C (beta) 键-Tyr-67-Gly-68 三肽。 带有发色团的肽的 MS 数据表明 …

Plasmid pSIN-PAmCherry-KFERQ-NE from Dr. Shu Leong Ho's lab contains the inserts PA-mCherry and KFERQ peptide and is published in Autophagy. 2019 Apr 14:1-24. doi: 10.1080/15548627.2019.1603545. This plasmid is available through Addgene.

PAmCherry is a photoactivatable fluorescent protein (PAFP) mutant of mCherry. Use PAmCherry to track cells, organelles, or proteins of interest against a dark background.

2009年12月15日 · Photoactivatable fluorescent proteins (PAFPs) are required for super-resolution imaging of live cells. Recently, the first red PAFP, PAmCherry1, was reported, which complements the photo-activatable GFP by providing a red super-resolution color. PAmCherry1 is originally "dark" but exhibits red fluorescence after UV-violet light irradiation.