
pCas9 - Addgene
Plasmid pCas9 from Dr. Luciano Marraffini's lab contains the insert tracr/Cas9 and is published in Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. This plasmid is available through Addgene.
pCas9-CR4 - Addgene
Plasmid pCas9-CR4 from Dr. Kristala Prather's lab contains the insert Cas9 nuclease and is published in Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096. This plasmid is available through Addgene.
pCas9 Sequence and Map - SnapGene
SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. The maps, notes, and annotations in the zip file on this page are copyrighted material.
CRISPR-based Genome Editing Systems For Editing in E. coli
In pCas9, we 1) replaced the promoter of gRNA-pMB1 with PrhaB, 2) changed the replicon of pCas into a non-temperature-sensitive replicon, and 3) added the sacB gene for counterselection to yield pEcCas.
A modified pCas/pTargetF system for CRISPR-Cas9-assisted ... - PubMed
2021年4月15日 · The clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we established previously has been widely used in Escherichia coli MG1655.
Establishment of an Integrated CRISPR/Cas9 Plasmid System for …
By using the enhanced green fluorescent protein reporter plasmid pGNtsf1, we demonstrate that pCas9-U6sgRNA driven by endogenous U6 promoter (pCas9-mU6sgRNA) mediated very high gene editing efficiency in medaka cultured cells, but not by exogenous U6 promoters.
pCas9 - Addgene
Plasmid pCas9 from Dr. Howard Hang's lab contains the inserts chloramphenicol acetyl transferase and pUC19 origin of replication and is published in Appl Environ Microbiol. 2021 Jul 7:AEM0084421. doi: 10.1128/AEM.00844-21. This plasmid is available through Addgene.
pCas Sequence and Map - SnapGene
Bacterial plasmid with a temperature-sensitive replicon encoding Cas9, an inducible single guide RNA (sgRNA), and the λ Red recombination system. The lac repressor binds to the lac …
The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome ...
2015年10月14日 · Cas9 is an endonuclease that targets a specific DNA sequence that can be easily programmed by a plasmid encoded target sequence of 20 bp 18. The only requirement of the Streptococcus pyogenes Cas9...
modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing ...
2021年3月25日 · The clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we established previously has been widely used in Escherichia coli MG1655.
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