Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC18. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends …

pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).

pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. [1] . The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the abbreviation for the University of California, where early work on the plasmid series had been conducted. [2] .

我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。 所有的产品都严格仅供科学研究使用，不能 …

Plasmid pUC19 from Dr. Joachim Messing's lab is published in Gene. 1983 Dec;26 (1):101-6. This plasmid is available through Addgene.

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence.

pUC19 载体是小分子量、高 拷贝的大肠杆菌质粒,长 度均为2,686 bp。 pUC19 质粒含有:(1)pMB1 复制子rep, 主管质粒复制( 来源pBR322 质粒)。 rop 基因缺失和pMB1的复制子rep 的单点突变是pUC 质粒高拷贝的原因;(2)bla 基因:编 码β- 内酰胺酶,对氨苄青霉素有抗性( 来源—pBR322 质粒)。 该基因有两个突变, 与pBR322 的bla 基因不同;(3)大 肠杆菌lac 操纵子区域含有CAP 蛋白结合位点、启 动子Plac、lac抑制子结合位点和lacZ 基因的5’- 端部分( 编码β- 半乳糖苷酶N- 部分( 来 …

pUC18 质粒载体与pUC19 质粒载体 除了 多克隆位点 （Multiple Clone Site）部分反向 互补 外，其它部分完全一样。 pUC18、pUC19载体适合于 DNA 片段的克隆、进行DNA测序、对 外 …

2023年8月3日 · It is a commonly used cloning vector in the bacteria E. coli. pUC19 is 2686 bp in length. The molecular weight of the pUC19 vector is 1.75×10 6 Da. It is a small plasmid with a …

pUC19载体适用于双脱氧法对DNA片段进行测序，它克隆的DNA片段比使用M13 phage作载体时克隆的片段长。 由于在lacZ领域中含有多克隆位点，因此可以在含有IPTG、X-Gal的平板培养基上通过蓝白筛选判断载体中有无DNA片段的插入。 同时还可以通过载体上的lac promoter表达外源基因，使用M13系列的通用引物对插入载体中的DNA片段进行测序。 使用Kilo-Sequence Deletion Kit (Code No.: 6030) 可以对克隆得到的长片段DNA进行测序。 -20℃。 使用CsCl-EtBr密度梯度超 …