Provide pDEST32 vector/plasmid map, full length sequence, antibiotic resistance, size and other information

The ProQuest™ Two-Hybrid System with Gateway™ Technology is an in vivo yeast-based system for identifying interactions between two proteins. This interaction reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media.

The ProQuest™ Two-Hybrid System includes yeast expression vectors pDEST™32, pDEST™22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase™ II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay.

The pDEST22/pDEST32 vectors produce a higher fraction of interactions that are conserved and that are biologically relevant when compared to the pGBKT7/pGADT7-related vectors, but the latter appear to be more sensitive and thus detect more interactions.

pDEST32 is available at Gentaur for Next week Delivery. Precision: The pDEST32 promotor is combined with a vector sequence and a gene in the plasmid vector map.. Assay procedure summary: Gentaur supplies 2ug of lyophilized DEST32 Plasmid in powder form at 4°C to be centrifuged at 5000rpm/min for 1min.

These baits were cloned into pDEST32 and screened against a yeast two-hybrid (Y2H) array of 1,704 S. pneumoniae TIGR4 ORFs cloned into prey vector pDEST22 as described 7 . The strength of Y2H...

The PDEST32 is a laboratory equipment designed for plasmid DNA extraction and purification. It utilizes a silica-based membrane technology to efficiently capture and purify plasmid DNA from bacterial cultures.

Use pDest32 for functional genomics research and drug discovery study.

Two Hybrid Vector Pdest32, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

Pdest32 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more