高效液相色谱法检测表面活性剂中聚乙二醇的含量 一、范围 本标准规定了用高效液相色谱法测定聚乙氧基化非离子表面活性剂中聚乙二醇（PEG）的方法该标准适用于测定聚乙氧基化脂肪醇和聚乙氧基化烷基酚中聚乙二醇的…

To develop and evaluate a two-dimensional LC system with charged aerosol detection for the simultaneous characterization of protein PEGylation and unreacted PEGylation reagent. The use of polyethylene glycol (PEG) as a safe and low cost additive for the pharmaceutical and cosmetic industries is common practice.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylene glycol, PEG 200, PEG 400. Polyethylene glycol (PEG) has a low toxicity and is used in a variety of products (creams, laxatives, lubricants). When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood.

本应用简报描述了一种联用Agilent HPLC 及Agilent 6520 精确质量数Q-TOF LC/MS 系统分析聚乙二醇及聚乙二醇化蛋白的方法。 其使用了一种 电荷剥离剂（三乙胺）作为柱后添加剂，这样有助于简化质谱图，因此可更好地阐释结 果。 结果表明聚乙二醇和聚乙二醇化蛋白质的质谱图质量均得到显著改善。 作者. R av in dr Gu h l 及S uresh Bab CV 安捷伦科技公司（印度） 印度班加罗尔 Ning Tang 安捷伦科技公司 美国加利福尼亚州圣克拉拉市 Sundaram Palaniswamy, …

1998年3月27日 · Derivatisation efficiency for PEGs by dinitrobenzoyl chloride and quantitative determination of derivatised PEGs by HPLC have been studied. A reversed-phase (RP) mode of HPLC was examined for determination of PEG 400 oligomers. The normal-phase system provided greater resolution for oligomers of PEGs. 1. Introduction.

HPLC is a powerful analytical technique that is widely used for the measurement of PEG, PEGylated small drugs, and PEGylated liposomes and micelles (Table 3). HPLC analysis of clinical samples usually requires pretreatment steps to reduce sample complexity.

1991年8月16日 · Although high-performance liquid chromatography (HPLC) is the technique of choice for the separation of ethoxylated oligomers, the subsequent detection and quantification has proved to be difficult. The insensitivity of these compounds towards conventional UV detection is due to the lack of chromaphoric structure.

2019年8月9日 · This application shows a method for HPLC-CAD analysis of polyethylene glycol (PEG). PEG is a polyether compound – a polymer of ethylene glycol. A number of PEGs exist based on molecular weight and geometry. PEG has many uses. In the food industry, PEG is used as an antifoaming agent, carrier, emulsifier, glazing agent and thickener.

本文建立了一种分析吐温 20 的RP-HPLC-ELSD 方法,该方法在直接进样 PEG 偶联的蛋白注射剂后,即可对其中的辅料吐温 20 进行准确的定量分析。 吐温 20 购自南京威尔药业股份有限公司(注射级);聚乙二醇(PEG,MW20000) 购自 JenKem Technology。 三氟乙酸 (TFA) 为 HPLC 级,购自 DikmaPure 公司;乙腈为 HPLC 级,购自 J.T. Baker 公司(美国)。 高纯水来自 Milli-Q 纯水系统(美国)现 制备。 含吐温 20 的PEG 偶联重组蛋白注射剂、不含吐温 20 的阴性样品和不含吐温 20 …

摘要:本文建立了聚乙二醇20K、40K、80K 的HPLC 测定方法。 采用色谱柱SHIMSEN Ankylo SEC-300分析,结果显示,PEG-OH-20K、PEG-OH-40K、PEG-OH-80K 各峰间的分离度大于1.5,峰形和重现性良好。 此方法可为聚乙二醇的分析提供参考。 关键词:聚乙二醇. SHIMSEN Ankylo SEC-300 . 1. 实验部分. SHIMSEN Pipet PMII-1000(P/N:380-00751-06)。 20K、PEG-OH-40K、PEG-OH-80K 各3 mg 的溶液。 [email protected]. 2. 实验结果. 3. 结论. 本文建 …