Vector for fusing EGFP to the C-terminus of a partner protein. For other reading frames, use pEGFP-N1 or pEGFP-N3.

pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized forbrighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

What is virus associated DNA, and why do I have to order it? Welcome to Vector Database! Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources. This vector is NOT available from Addgene and the database is no longer actively maintained. This vector is not available from Addgene.

pegfp-n2是一种优化的突变型gfp，使其产生的荧光较普通gfp强35倍，大大增强了其报告基因的敏感度。egfp与其他蛋白的融合表达已有很多成功的例子，而且其n及c端均可融合，并不影响其发光。

提供 pegfp-n2 载体/质粒图谱，全长序列，抗性，大小和元件信息。

Plasmid pEGFP from Dr. Koen Venken's lab contains the insert EGFP and is published in Cell Rep. 2021 Sep 14;36(11):109700. doi: 10.1016/j.celrep.2021.109700. This plasmid is available through Addgene.

Plasmid Emerin pEGFP-N2 (588) from Dr. Eric Schirmer's lab contains the insert Emerin and is published in J Cell Biol. 2011 Apr 4;193 (1):109-23. doi: 10.1083/jcb.201009068. Epub 2011 Mar 28. This plasmid is available through Addgene.

pEGFP-N2-NRSE4x-ohne-CMV 载体 (V007595) 我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。

pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

2022年2月16日 · pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.