Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28a (+) or pET-28c (+). Sequence Author: MilliporeSigma …

Its T7 promoter system, 6xHis-tag, and Kanamycin resistance gene provide strong transcription, easy purification, and selection in E. coli. pET-28b+ Plasmid DNA is a valuable tool for molecular cloning, subcloning, mutagenesis, and site-directed mutagenesis, and is widely used in protein expression and purification research.

Provide pET-28b (+) vector/plasmid map, full length sequence, antibiotic resistance, size and other information

"The maps for pET-28b (+) and pET-28c (+) are the same as pET-28a (+) (shown) with the following exceptions: pET-28b (+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at 198....

pET-28b (+) DNA - Novagen Novagen's pET-28a-c (+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag® configuration plus an optional C-terminal His•Tag sequence. - …

The pET-28b (+) expression vector used as the template for plasmid modification was obtained from Novagen. S. cerevisiae strain BJ5464-NpgA (MATα ura3-52 his3-Δ200 leu2-Δ1 trp1 pep4∷HIS3 prb1 Δ1.6R can1 GAL) was used for the recombinatorial assembly of PCR products (Ma et al., 2009).

2011年8月25日 · The pET-28b (+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. The T7 expression region is reversed on the circular map due to the sequence numbering from the pBR322 convention.

Plasmid pET-28b-Cas9-His from Dr. Alex Schier's lab contains the insert Cas9 and is published in PLoS One. 2014 May 29;9 (5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014. This plasmid is available through Addgene.

Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b (+) or pET-28c (+).

The pET-28a-c (+) vectors carry an N-terminal HIS TAG ® /thrombin/T7•Tag configuration plus an optional C-terminal HIS TAG sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB074). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded …