pet-46 ek/lic载体是用来快速定向pcr高通量质粒构建的载体。 本载体带有N-Nus标签和His标签，S标签。 独特的酶切位点在载体图谱中被标注出来。

pET-46 Ek_LIC Bacterial vector for ligation-independent cloning (LIC) to express 6xHis-tagged proteins with an enterokinase site. Sequence Author : MilliporeSigma (Novagen)

The pET-46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for highlevel expression of polypeptides. Using specifically designed primers for amplification and the pET-46 Ek/LIC Cloning Kit (Cat. No. 71335-3), inserts can be efficiently cloned without the need for restriction digestion or ligation.

2023年9月11日 · Here we report on the structural and biochemical characterization of PET46 (RLI42440.1), an archaeal promiscuous feruloyl esterase exhibiting degradation activity on semi-crystalline PET powder...

2023年9月25日 · Using a metagenomic approach, the research team has identified and biochemically described the PET-degrading enzyme PET46 from a non-cultured deep-sea microorganism for the first time.

The pET-46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for highlevel expression of polypeptides. Using specifically designed primers for amplification and the pET-46 Ek/LIC Cloning Kit (Cat. No. 71335-3), inserts can be efficiently cloned without the need for restriction digestion or ligation.

The pET-46 Ek/LIC Vector is a LIC-compatible derivative of pET-45b(+). The plasmid contains a strong T7lac promoter and an amino-terminal HIS TAG ® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence.

pET46 EK/LIC 载 体 简 介 The pET-­‐46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-­‐amplified DNA for highlevel expression of polypeptides. Using specifically designed primers for amplification and the pET-­‐46 Ek/LIC Cloning Kit (Cat.

2023年9月28日 · PET46 has unique properties, such as the ability to degrade long-chain and short-chain PET molecules, making it valuable for biotechnology applications. This research is part of the PLASTISEA project and sheds light on the potential for plastic waste degradation in the sea and on land.

2025年3月10日 · ChemicalBook 致力于为化学行业用户提供PET-46 EK/LIC 载体的性质、化学式、分子式、比重、密度,同时也包括PET-46 EK/LIC 载体的沸点、熔点、MSDS、用途、作用、毒性、价格、生产厂家、用途、上游原料、下游产品等信息。