pET-22b(+) Sequence and Map - SnapGene
Bacterial vector that encodes a signal sequence for inducible expression of proteins in the periplasm.
pET-22b(+) pet22b质粒图谱、载体图谱、序列、价格、抗性、测 …
pET-22b (+)载体 (货号：69744-3)携带有一个N端的pelB信号肽序列，能够将表达的目的蛋白定位于外周质腔，同时载体含有C端His标签。 载体的单一的多克隆位点见上面的环状质粒图谱。 注意：载体序列是以pBR322质粒的编码规矩进行编码的，所以T7蛋白表达区在质粒图谱上面是反向的。 T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。 质粒的F1复制子是 …
Addgene: Vector Database - pET-22b(+)
PelB sequence for periplasmic localization Bacterial vector for expressing proteins in the periplasm. How was your experience today? Addgene is a nonprofit plasmid repository.
The pET-22b(+) vector (Cat. No. 69744-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map.
pET-22b (+) vector map and sequence
Buy one, get one free! Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg. 1. Centrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4.
69744 Sigma-Aldrich pET-22b(+) DNA - Novagen - Merck
pET-22b (+) DNA - Novagen The Novagen pET-22b (+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag sequence. - Find MSDS or SDS, a COA, data sheets and more information.
pET-22b(+) DNA, Novagen, with N-terminal pelB sequence Sigma-Aldrich
pET-22b(+) DNA - Novagen Novagen pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization; Synonyms: Novagen DNA Vector,cloning vector,expression vector,pET-22b(+) DNA,pET-22b(+) Vector,vector at Sigma-Aldrich
pET-22b(+) · Benchling
Full sequence for pET-22b (+) shared on Benchling.
Periplasmic production via the pET expression system of soluble ...
The pET22b plasmid was used as a template for creating vectors that encode hGH fused to ... A pET based expression system for the production of recombinant human growth hormone (hGH) directed to the Escherichia coli periplasmic space was developed.
Vector: pET-22b(+) | Genophore
The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB038). Source: Sigma-Aldrich .