3 天之前 · Phospho-GCN2 (Thr899) (E1V9M) Rabbit mAb recognizes endogenous levels of GCN2 protein only when phosphorylated at Thr899. The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST.

The serine/threonine-protein kinase general control nonderepressible 2 (GCN2) is a well-known stress sensor that responds to amino acid starvation and other stresses, making it critical to the maintenance of cellular and organismal homeostasis.

2023年9月8日 · Here we find that the widely used ATP-competitive inhibitors of PERK, GSK2656157, GSK2606414 and AMG44, inhibit PERK in the nanomolar range, but surprisingly activate the ISR via GCN2 at micromolar...

2016年11月22日 · GCN2 is one of four “stress kinases” that block translation by phosphorylating eIF2α. GCN2 is thought to bind uncharged tRNAs to “sense” amino acid availability. In mammals, myeloid cells expressing indoleamine dioxygenases locally deplete tryptophan, which is detected by GCN2 in T cells to cause proliferative arrest.

2023年9月20日 · GCN2是在所有真核生物中发现的最进化保守的eIF2AK，其功能是通过调节细胞类型特异性功能来调节生理和病理进程，包括代谢、免疫、癌症等。 与此同时一些证据表明GCN2在调节巨噬细胞（Mφ）功能中起着关键作用，GCN2的缺失导致腹膜Mφ对dsDNA病毒感染的易感性增加。 Mφ在调节红细胞（RBCs）的产生和清除之间的平衡方面也发挥着核心作用，尤其对于在急性失血或溶血等应激条件下促进红细胞的生产。 红细胞在骨髓（BM）中的红细胞 …

GCN2 activation in T cells leads to an induction of the integrated stress response pathway and subsequently to T cell anergy and apoptosis. Here, we demonstrate that the pharmacologic inhibition of GCN2 restores the T cell proliferation and effector function in amino-acid, glucose-deficient media and in MDSC-induced T cell suppression.

2019年3月14日 · We describe here experiments that identify a common downstream effector mechanism of T cell inhibition in both human and mouse MSCs as Endoplasmic Reticulum (ER) stress. In human T cells this inhibition is mediated by IDO depletion of tryptophan acting in a quantal manner to produce an “all-or-nothing” switch at tryptophan concentrations ...

GCN2 (general control nonderepressible 2) 是一种应激反应激酶，在各种应激源下在维持细胞稳态中起关键作用。GCN2 (pGCN2) 响应压力的磷酸化导致整体蛋白质合成的抑制，并随后导致 1) T 细胞无反应和细胞凋亡，2) 增强的 MDSC 依赖性免疫抑制和 3) 肿瘤细胞存活。

3 天之前 · Phospho-GCN2 (Thr898) Antibody detects endogenous levels of GCN2 only when phosphorylated at threonine 898. Human, Mouse, Rat. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr898 of murine GCN2. Antibodies are purified by protein A and peptide affinity chromatography.

pG2-er载体价格6800元，pG2-er载体质粒图谱(Vector map)，载体序列(Sequence)见下文，测序引物为见下文。