
pGFP Sequence and Map - SnapGene
Vector for expressing GFP in bacteria. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. Sticky ends from different BspQI sites may not be compatible. Sticky ends from different SapI sites may not be compatible.
pGFP - Addgene
Plasmid pGFP from Dr. Stephen Mayfield's lab contains the insert Green fluorescent protein and is published in AMB Express. 2015 Dec;5(1):126. doi: 10.1186/s13568-015-0126-3. Epub 2015 Jul 3. This plasmid is available through Addgene.
pGFP 载体图谱和序列 - 纽普生物
提供 pGFP 载体/质粒图谱,全长序列,抗性,大小和元件信息。
pGFP-V-RS 载体图谱和序列 - 纽普生物
提供 pgfp-v-rs 载体/质粒图谱,全长序列,抗性,大小和元件信息。
pGFP carries the complete GFP coding sequence derived from the GFP cDNA by PCR (1, 2). This PCR product was cloned between the two MCSs of the pUC19 derivative pPD16.43 (2, 5). The 5' MCS lies immediately upstream from the GFP start codon; the 3' MCS lies downstream from the GFP stop codon.
pEGFP-C1 pEGFPC1质粒图谱、载体图谱、序列、价格、抗性、测 …
pEGFP-C1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-C1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.
An improved green fluorescent protein gene as a vital marker in …
A synthetic green fluorescent protein (GFP) gene (pgfp) was constructed to improve GFP expression in plants. Corn and tobacco protoplast transient assays showed that pgfp gave about 20-fold brighter fluorescence than the wild-type gene (gfp).
pEGFP-N1 Sequence and Map - SnapGene
Vector for fusing EGFP to the C-terminus of a partner protein. For other reading frames, use pEGFP-N2 or pEGFP-N3. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k Plasmids: …
pEGFP-N1 载体图谱和序列 - 纽普生物
The pEGFP-N1 is a vector for fusing EGFP to the C-terminus of a partner protein. It has multiple cloning sites before the GFP gene. 1. 发货形式:质粒干粉 (常温运输,存于-20度,请务必先转化提质粒后使用) 2. 收到质粒干粉后请先5000rpm离心1min,再加入20μl ddH2O溶解质粒;(质粒复测的浓度有时候与标称值差距较大,这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致,因此建议先转化提质粒后再使用) 3.
pEGFP-N1载体 - 百度百科
绿色荧光蛋白 (Green Fluorescent Protein,GFP)基因来源于Jellyfish Aequorea Victoria, 是Shimomura等于1962年发现的蛋白质,由238个氨基酸组成,分子量约为27Kd。 GFP在包括热、极端PH和化学变性剂等苛刻条件下都很稳定,用甲醛固定后会持续发出荧光,但在还原环境下荧光 …