Lmless marrow cells are made available presumably to repair an essential antigen-trapping apparatus disrupted by the irradiation. Another interpretation is that thymus lymphocytes lack...

DNA sequence analysis of the polymerase chain reaction-amplified beta-globin gene revealed a point mutation (T --> C) in the second nucleotide of the 42nd codon of the beta-globin chain (beta 42 (CD1) Phe --> Ser). Hemoglobin Hammersmith, a rare, unstable hemoglobin variant, was diagnosed in a 9-year-old Japanese girl.

The distal Arg E7 (red) is oriented toward the CD corner, disturbing the position of Phe CD1 and Phe CD4 (orange). The heme pocket entrance is much wider allowing increased access to ligands. However, unlike normal His E7, mutant Arg is unable to …

2005年8月2日 · Hemoglobin Hammersmith, a rare, unstable hemoglobin variant, was diagnosed in a 9-year-old Japanese girl. She presented with the typical manifestations of this disorder, including neonatal hyperbilirubinemia, followed by progressive hepatosplenomegaly, jaundice, and bilirubinuria.

2006年2月10日 · Phe CD1 occurs in conjunction with a H-bond donor at the E11 position (Gln or Ser), whereas His CD1 is accompanied by a hydrophobic residue (Leu or Phe). This analysis offers four possible configurations for ligand interactions: Tyr CD1 and hydrophobic E11, His CD1 and hydrophobic E11, Phe CD1 and Gln E11, and Phe CD1 and Ser E11.

1999年8月20日 · Among the hydrophobic heme-binding residues one (Phe CD1) is identical in all sequences, while another, FG5, is aliphatic in ⩾95% sequences in each subfamily. In contrast, five hydrophobic residues, which are near to the heme but do not bind it, can be occupied by either aliphatic or aromatic residues and only one, H19, is occupied mainly by ...

1973年4月16日 · L'Hémoglobine Hammersmith β 42 (CD 1) Phe → Ser est une hémoglobine instable à affinité pour l'oxygène diminuée. Des études faites sur des globules rouges intacts ont motré que le principal mécanisme compensateur de cette diminution d'affinité pour l'oxygène est un changement de pH avec effet Bohr, et que le 2.3 DPG n'est que peu ...

The structural abnormality was identified as a replacement of phenylalanyl residue in position 42 (CD1) by a leucyl residue. Substitution of this phenylalanyl residue, which participates in the contact with heme, by a nonpolar leucyl residue has apparently less severe consequences than a replacement of the same residue by a polar seryl residue ...

1990年1月5日 · In Hb Warsaw Val replaces the Phe normally present at the heme contact position beta 42 (CD1). This variant is unstable, and it readily undergoes methemoglobin formation. In DEAE-cellulose chromatography, the variant hemoglobin co-eluted with Hb A; a partially heme-depleted fraction of the variant, …

1996年10月18日 · Functional studies of partially purified hemoglobin (Hb) Bruxelles, Phe β42 (CD1) → 0 indicate a major shift in the allosteric equilibrium toward the deoxy (T state) conformation. While Hb A shows a roughly symmetrical oxygenation curve with maximum cooperativity near half-saturation, Hb Bruxelles shows mainly properties of the low affinity ...