Here, we explore how PI (3,5)P 2 levels within cells are regulated. We find the PIKfyve complex comprises five copies of the scaffolding protein Vac14 and one copy each of the lipid kinase …

2010年7月13日 · In this paper, by direct patch-clamping of the endolysosomal membrane, we report that PI (3,5)P 2, an endolysosome-specific PIP, binds and activates endolysosome …

Phosphatidylinositol 3,5-bisphosphate is in very low abundance and is required for retrograde membrane trafficking from lysosomal and late endosomal compartments to the Golgi and is …

2023年9月4日 · Using the amoeba Dictyostelium discoideum, we identify SnxA as a highly selective PI (3,5)P2-binding protein and characterize its use as a reporter for PI (3,5)P2 in both …

Here, using biochemical characterization of isolated yeast vacuolar vesicles, we demonstrate that addition of exogenous short-chain PI (3,5)P2 to Vph1-containing vacuolar vesicles activates V …

2008年9月11日 · With the PI response to cell stress, in their seminal paper identifying PtdIms 3,5‐bisphosphate (PI35P2) as a novel endogenous PI species, Dove et al reported that …

TORC1, a conserved protein kinase, regulates cell growth in response to nutrients. Localization of mammalian TORC1 to lysosomes is essential for TORC1 activation. Phosphatidylinositol 3,5 …

2023年8月14日 · Since its discovery in 1997 (2), PI (3,5)P 2 has become one of the most enigmatic lipid pathways in animal cells but has also shown great promise for clinical …

Here, we identify the branched actin regulator cortactin as a direct binding partner of phosphatidylinositol 3,5-bisphosphate (PI (3,5)P 2) and demonstrate that their interaction …

Although the cellular levels of phosphatidylinositol 5-phosphate (PI5P) and phosphatidylinositol 3,5-bisphosphate (PI (3,5)P 2) are extremely low relative to some other PIs, emerging …