We used pig-mouse cytoplasmic hybrids as a model to address nuclear-cytoplasmic incompatibility in iSCNT embryos. Fertilized murine zygotes were reconstructed by fusing with porcine cytoplasts of varying cytoplasmic volumes (1/10 …

2017年11月10日 · In pigs, supplementation of in vitro maturation (IVM) culture with lanosterol improved preimplantation development of SCNT embryos, possibly by elevating lipid content of oocytes or regulating genes involved in the cholesterol biosynthetic pathway in cumulus cells or those involved in lipid metabolism and apoptosis, with a positive effect on ...

This study explored the possibility of producing transgenic cloned embryos by interspecies somatic cell nuclear transfer (iSCNT) of cattle, mice, and chicken donor cells into enucleated pig oocytes. Enhanced green florescent protein (EGFP)-expressing donor cells were used for the nuclear transfer.

Interspecies somatic cell nuclear transfer (iSCNT) has an immense potential to rescue endangered animals and extinct species like mammoths. In this study, we successfully established an Asian elephant's fibroblast cell lines from ear tissues, performed iSCNT with porcine oocytes and evaluated the …

2021年4月6日 · In this study, we investigated if 50 nM TSA treatment for 24 h would improve the rate of embryo development to the blastocyst stage in cat-pig iSCNT embryos, compared with cat-cow iSCNT embryos under same treatment.

摘要： 食蟹猴-猪异种体细胞核移植(Interspecies somatic cell nuclear transfer, iSCNT)研究旨在由iSCNT胚胎建立具有与人类相似遗传背景的胚胎干细胞 (ESCs), 作为医学和基础科学研究的实验材料。文章探讨了两种组蛋白脱乙酰化酶抑制剂(HDACi)-Trichostatin A(TSA)和Valproic acid(VPA ...

2014年1月1日 · Domestic animals, such as cows, pigs, and rabbits – probably the best sources so far – have been used widely (Beyhan et al., 2007, Loi et al., 2011, Gomez et al., 2009). Other species that have served as iSCNT oocyte donors include goats, sheep, wild and domestic cats, dogs, and mice (Beyhan et al., 2007) (Table 35.1).

2020年10月1日 · As a proof-of-concept that the quantity of material, rather than its species of origin, can influence the iSCNT experiment outcomes, we tested whether porcine NLBs transferred to mouse enucleolated eggs, can rescue the development if the amount of the NLB material is adjusted with respect to the embryo species of origin.

2011年7月15日 · Much emphasis is currently given to the use of Interspecific Somatic Cell Nuclear Transfer (ISCNT) as a potential salvage tool for endangered animals. In this short review we present a survey on all data published so far on ISCNT, including abstract communication in international meetings.

Using a bovine-pig (inter-family) iSCNT model, we studied the basic characteristics of EGA: expression and activity of RNA polymerase II (RNA Pol II), formation of nucleoli (as an indicator of RNA polymerase I (RNA Pol I) activity), expression of the key pluripotency gene NANOG and alteration of mitochondrial mass.