2020年11月4日 · Liquid-liquid phase separation (LLPS) of proteins that leads to formation of membrane-less organelles is critical to many biochemical processes in the cell....

2022年1月15日 · Liquid-liquid phase separation (LLPS) is associated with neurodegenerative diseases. PrP can undergo LLPS in the absence and the presence of nucleic acids. The N-terminal intrinsically disordered region of PrP is the primary driver of LLPS. LLPS might offer an alternative non-canonical pathway to promote PrP aggregation.

2021年8月2日 · Emerging evidence suggests that liquid–liquid phase separation (LLPS) represents a vital and ubiquitous phenomenon underlying the formation of membraneless organelles in...

2023年7月11日 · In the model plant, Arabidopsis thaliana, LLPS by the protein EARLY FLOWERING3 (ELF3) occurs in a temperature-sensitive manner and controls thermoresponsive growth. ELF3 contains a largely unstructured prion-like domain (PrLD) that acts as a driver of LLPS in vivo and in vitro.

液-液相分离（Liquid-Liquid Phase Separation, LLPS）是指在细胞内某些生物大分子（如蛋白质和RNA）通过相互作用，形成具有不同组分和性质的相分离液滴 [1]。 这些液滴类似于油滴在水中的分离状态，形成了细胞内的…

2018年12月5日 · Here, we describe a step-by-step protocol commonly used to investigate in vitro LLPS using purified proteins. This protocol includes expression and purification of the studied proteins, induction of LLPS of the purified proteins, and studies of the biophysical properties of the liquid droplets formed by LLPS.

Phase separation, or liquid-liquid phase separation (LLPS), is a ubiquitous and important mechanism for the formation of membraneless condensates in cells. LLPS is driven by multivalent macromolecular interactions, whereas resulting condensates have been regarded as an emerging paradigm of cellular organization (Banani et al., 2017; Boeynaems ...

2020年10月18日 · Our analyses reveal that metal-coordinated virus NC protein PrLDs initiate LLPS that nucleate pan-virus assembly and contribute to their persistence as cell-free infectious aerosol droplets.

2021年10月21日 · Su et al. review the broad impact of liquid-liquid phase separation (LLPS) on various cellular processes and signaling pathways, emphasizing the spatiotemporal regulation of cell signaling by LLPS and the underlying molecular mechanisms involved.

2020年3月27日 · Liquid–liquid phase separation (LLPS) of proteins underlies the formation of membrane-less organelles. While it has been recognized for some time that these organelles are of key importance for normal cellular functions, a growing number of recent observations indicate that LLPS may also play a role in disease.