piRNA （PIWI-interacting RNA），是一种长25～33nt的非编码RNA，通过与 PIWI蛋白 相互作用而发挥作用，虽然同为非编码RNA，piRNA却远没有miRNA、LncRNA、 circRNA 知名度高，主要是因为piRNA主要在生殖系统中表达，相关研究也大多集中于此。 最近piRNA相关的研究逐渐增多，piRNA研究工作登上 CNS 的次数比最近大热的circRNA还要多得多。 piRNA与基因组稳定性保持、表观遗传学调节、生殖干细胞分化、胚胎发育和多种疾病的发生、发展密切相关。 PIWI蛋 …

2020年4月15日 · Piwi-interacting RNAs (piRNAs) have been linked to epigenetic and post-transcriptional gene silencing of retrotransposons in germ line cells, particularly in spermatogenesis. Exosomes are important mediators of vesicle transport, and the piRNAs in exosomes might play an important role in cell communication and signal pathway regulation.

2024年9月1日 · Furthermore, we focus on the expression pattern of PIWI genes at the RNA and protein levels in renal cells. We show that, despite all four Piwi RNAs are detected by qRT-PCR, only Piwil1 protein is monitored by western blotting.

PIWI-interacting RNAs (piRNAs) are a novel type of small non-coding RNAs (sncRNAs), which are 26–31 nucleotides in length and bind to PIWI proteins. Although piRNAs were originally discovered in germline cells and are thought to be essential regulators for germline preservation, they can also influence gene expression in somatic cells.

The expression patterns of PIWIL1, PIWIL2, PIWIL3, PIWIL4 mRNA, was assessed via real-time quantitative polymerase chain reaction (RT-QPCR), on tissue and serum samples from HCC patients, their impact for diagnosis was evaluated by ROC curves, prognostic utility was determined, and In Silico analysis was conducted for predicted variant ...

2019年3月1日 · Piwi-interacting RNA (piRNA), a class of noncoding RNAs, is a potential biomarker for body fluid identification because of its short length (˜24–32 nt) and specific expression pattern in human tissues.

(B) qPCR of PIWI/AGO transcripts in Aag2 cells at 48 h after transfection of control dsRNA (dsLuc) or dsRNA targeting the corresponding gene. Expression levels were normalized to the control ...

2018年1月10日 · In this study, we investigated the expression of PIWILs by qRT-PCR and western blotting and then identified piRNA transcriptomes in tissues of normal ovary and two most prevalent epithelial ovarian cancer subtypes, serous and endometrioid by small RNA sequencing.

In this proof-of-principle study, we examined the expression levels of four carefully selected piRNAs in forensically relevant biological fluids (venous blood, saliva, semen, menstrual blood and vaginal secretions) using TaqMan quantitative real-time polymerase chain reaction (TaqMan qPCR). piR-55521, which was not detectable in saliva, can ...

PIWI相互作用的RNA piR-13643和piR-21238是甲状腺乳头状癌的有前途的诊断生物标志物。 新兴研究表明，PIWI相互作用RNA（piRNA）参与了癌症的发展。 该三相生物标志物鉴定研究包括75对甲状腺乳头状癌（PTC）样品和31例甲状腺良性结节样品。