Eleven serotypes of AAV have thus far been identified, with the best characterized and most commonly used being AAV2. These serotypes differ in their tropism, or the types of cells they infect, making AAV a very useful system for preferentially transducing specific cell types.

2015年3月30日 · Plasmids are important “vehicles” for the communication of genetic information between bacteria. The exchange of plasmids transmits pathogenically and environmentally relevant traits to the host bacteria, promoting their rapid evolution and adaptation to …

Recombinant AAV gene therapy vectors (rAAV) can be made by cotransfection of 293 cells by two plasmids. The first plasmid contains the rAAV sequences and consists of the therapeutic expression cassette between AAV terminal repeats.

Our AAV Rep-Cap plasmids work with our AAV Helper Free Systems to produce recombinant AAV more safely and easily, without the need for a helper virus. Choose one of these plasmids if you already have the AAV Helper Free System and would like to try a different AAV serotype.

2020年12月3日 · You just need three plasmids to start making the AAVs you need for your experiment: and the transfer plasmid which contains the viral genome. In today’s blog post, we’ll focus on the packaging plasmid and take a look at each of its parts.

2003年6月1日 · Cross-packaging of vector DNA derived from AAV-2, -3, or -6 was up to 10-fold more efficient using our novel plasmids, compared to a conservative adenovirus-dependent method. We also identified a variety of useful antibodies, allowing detection of Rep or VP proteins, or assembled capsids, of all six AAV serotypes.

Today, plasmids are widely recognized not only as important factors facilitating genome restructuring but also as vehicles for the dissemination of beneficial characters within bacterial communities.

2024年9月13日 · DBP binds to single stranded DNA in a sequence independent manner and functions to increase the processivity of AAV DNA replication by stimulating elongation and stabilizing the displaced ssDNA from nuclease degradation. 23–25 In addition, DBP has regulatory roles in enhancing AAV p5 promoter activity …

1999年10月1日 · In this report we describe new helper plasmids (pSH3 and pSH5) that eliminate the Ad co-infection requirement. The helper plasmids express the AAV rep and cap genes and the Ad E2A, VAI and E4 genes.

2007年1月1日 · Linear plasmids of eukaryotic microbes are contemporary manifestations of ancient viruses, which have adjusted to two cellular compartments during evolution, i.e., to the mitochondrium or to the cytoplasm. In either case, infectious viral …