2022年6月22日 · Our results indicate that the poly (A) capture full-length double-strand cDNA sequencing improves transcript quantification accuracy and the detection ability of alternative …

PolyA信号，也被称为聚腺苷酸化信号或 polyA尾，是真核基因3'末端普遍存在的信号序列。 其核心序列基序是 AAUAAA，其中AATAAA核心基序是最早被鉴定出来的polyA信号，对mRNA 3'末 …

2023年4月15日 · Illumina RNA-Seq的原理是通过富集mRNA（polyA富集）或去除rRNA，产生片段化的RNA序列，然后将RNA逆转录生成双链cDNA，在片段末尾加入测序接头后进行PCR扩 …

In this method paper, we present a new NGS-based method Poly(A)-seq for global profiling of poly(A) tails and measured poly(A) tail lengths, which includes the experimental method for …

2022年6月22日 · Our results indicate that the poly (A) capture full-length double-strand cDNA sequencing improves transcript quantification accuracy and the detection ability of alternative …

Polyadenylation is the process by which nontemplated adenosines are added to the 3′‐end of mRNA, which occurs posttranscriptionally in the nucleus and is performed by canonical poly …

Here we describe a protocol that couples poly(A) polymerase with a multifunctional reverse transcriptase (RT) for targeted cloning of environmental prokaryotic cDNA. In essence, …

2019年11月22日 · Here, we develop a method at single-cell level sensitivity that enables quantification of poly (A) tails along with the full-length cDNA while reading non-adenosine …

我们的结果表明，poly(A) 捕获全长双链 cDNA 测序提高了转录本量化的准确性和选择性剪接事件的检测能力。 它还有望有助于确定 DNA 变体对剪接事件的重要性。

Poly(A)-seq is a common method for expression profiling and transcript quantification in a sample under specific conditions. Researchers can cheaply and quickly identify novel transcripts and …