2012年3月1日 · Paramagnetic Relaxation Enhancement (PRE) is increasingly becoming a method to provide long-range distance information that can complement NOE restraints, which are limited to distances of less than 6A. This method can be used to get distances in the range of 15-24 A (for a lone spin of a nitroxide stable radical).

2018年9月15日 · Paramagnetic relaxation enhancement (PRE) has been established as a powerful tool in NMR for investigating protein structure and dynamics. The PRE is usually measured with a paramagnetic probe covalently attached at a specific site of an otherwise diamagnetic protein.

In this brief review, we summarize various aspects of NMR paramagnetic relaxation enhancement (PRE). We discuss the types of spin labels used in NMR studies, describe the relevant theory used to accurately calculate PREs from coordinates, including how to take into account the fact that paramagnetic …

2022年4月6日 · Here, we show that metallohost–guest binding and exchange in a paramagnetic manganese (III) porphyrin macrocycle can be studied by NMR spectroscopy by exploiting the paramagnetic properties of...

然而磁场中顺磁离子的存在会产生顺磁弛豫增强（Paramagnetic Relaxation Enhancement, 简记为PRE）作用，即单电子与原子核的偶极—偶极相互作用加快了原子核的弛豫速率。

2015年1月1日 · In this chapter, we aim to prepare the typical NMR spectroscopist with the essential knowledge for using sPRE by outlining the basic theory, the various considerations prior to setting up sPRE experiments, the implementation of sPRE pulse programs and the processing and interpretation of sPRE data.

NMR interchain paramagnetic relaxation enhancement (PRE) techniques are a very powerful approach for detecting transient interchain interactions between intrinsically disordered proteins. These experiments, requiring a mixed sample containing a 1:1 ratio of isotope-labeled 15 N protein and natural abundance 14 N protein with a paramagnetic spin ...

2019年10月12日 · Paramagnetic relaxation enhancement (PRE) can be used to determine long-range distance restraints in biomolecules. The PREs are typically determined by analysis of intensity differences in HSQC experiments of paramagnetic and diamagnetic spin labels.

2013年12月12日 · More recently, paramagnetic relaxation enhancement (PRE) NMR proved to be an extremely powerful tool for solution structure determination but also to probe dynamics in biological macromolecules. 9−11 PRE NMR is based on the introduction of a paramagnetic center, for example, an unpaired electron with an isotropic g-tensor like in nitroxide ...

2001年3月19日 · It is shown that the electron spin motion is the determining factor of the properties of the NMR-PRE. The discussion is divided into three sections. Section 1 develops an intuitive physical description of the relaxation mechanism that is based on semi-classical pictures of the spin motion derived from the spin wavefunctions and level diagram.