本文介绍了一种利用新发现的 DNA 脱氨酶进行非破坏性 5-甲基胞嘧啶测序的方法（SEM-seq）。 最新版本的 NEBaseChanger在线工具有助于设计定点突变引物，兼容模糊碱基，根据氨基酸位置进行替换突变，批量上传多个引物组，并在引物尾部引入突变。 我们最新一期的 NEBExpression 涵盖了用于受监管市场的冻干分析产品、最新的 phi29-XT RCA（滚环扩增）试剂盒、Web 工具更新以及用于自动化 DNA 组装、测序、突变、无细胞蛋白表达等的解决方案！

For 15 years, NEB has addressed these challenges by offering solutions that streamline sample prep workflows, minimize inputs and improve library yield and quality. With over 100 products, the NEBNext portfolio includes sample prep solutions for DNA sequencing and RNA sequencing from a wide range of sample types.

The NEBNext UltraExpress DNA Library Prep Kit is the latest generation of NEBNext DNA library prep, with a fast, streamlined workflow. In under 2 hours, the protocol enables the creation of high-yield, high-quality libraries, from a broad input …

Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. Select the product group of the polymerase or kit you plan to use. Select the …

NEBuilder® HiFi DNA Assembly or Gibson Assembly® reactions. Need assistance setting up your NEBuilder protocol and assembly reaction? Use NEBuilder Protocol Calculator to generate …

The NEBNext ® Ultra™ II solution for Illumina DNA library prep is a streamlined suite of reagents and workflows designed for the construction of high-quality libraries, using input amounts from pg to μg of DNA. Workflows are fast, user-friendly, and automatable.

This paper proposes a web-based platform for scoring potential immunological reagents based on the structures or 3D models of the proteins of interest. The method scores a protein's peptides set, which is derived from a sliding window, based on the average solvent exposure, with a filter on the average local model quality for each peptide.

g genetic disorders. Red/ET Recombination enables in vivo DNA modifications irre-spective of . omposition and size. Thus, Gene Bridges ́ recombi-nation kits open up exciting possibilities for the fast and reliable engineering of . argeting constructs. Alternatively, why not take advantage of our service facility in . yte microinjection.

2025年3月10日 · For over 40 years, NEB has provided scientists with high-quality reagents and customized solutions. We offer larger quantities of catalog products, custom formulations and packaging, and lyophilization to meet unique needs.

Get started designing primers. NEB LAMP Primer Design Tool can be used to design core and loop primers for your LAMP reactions. 1. Input source sequence. Subsequence used must be …