An unstructured 5′-coding region of the prfA mRNA is required …
2011年11月2日 · In vitro transcription/translation and mutational analysis suggests a role for the first 20 codons of the native prfA -mRNA for maximal expression. By toe-print and RNA …
A Tale of Two Transitions: The Unfolding Mechanism of the prfA …
2020年11月24日 · RNA thermosensors (RNATs), found in the 5′ untranslated region (UTR) of some bacterial messenger RNAs (mRNAs), control the translation of the downstream gene in …
PrfA thermoregulator UTR - Wikipedia
The PrfA thermoregulator UTR is an RNA thermometer found in the 5' UTR of the prfA gene. [1] In Listeria monocytogenes, virulence genes are maximally expressed at 37 °C (human body …
An RNA Thermosensor Controls Expression of Virulence Genes in
2002年9月6日 · In Listeria monocytogenes, virulence genes are maximally expressed at 37°C, almost silent at 30°C and controlled by PrfA, a transcriptional activator whose expression is …
The PrfA virulence regulon - ScienceDirect
2007年8月1日 · Regulation operates through changes in PrfA activity—presumably by cofactor-mediated allosteric shift—and concentration, and involves transcriptional, translational and …
A trans -Acting Riboswitch Controls Expression of the Virulence ...
2009年11月13日 · Here, we demonstrate that two S -adenosylmethionine (SAM) riboswitches, SreA and SreB, can also function in trans and act as noncoding RNAs in Listeria …
Bacterial RNA thermometers: molecular zippers and switches
2012年3月16日 · Structured regions in bacterial mRNAs can act as thermosensors, known as RNA thermometers (RNATs), that control translation efficiency of the transcript. Some RNATs …
RNA-Mediated Control in Listeria monocytogenes - PubMed …
The master coordinator of transcription of the virulence genes of L. monocytogenes is transcriptional regulator PrfA (positive regulatory factor A), which belongs to the superfamily of …
An unstructured 5'-coding region of the prfA mRNA is required …
In vitro transcription/translation and mutational analysis suggests a role for the first 20 codons of the native prfA-mRNA for maximal expression. By toe-print and RNA-probing analysis, a …
Regulation of Listeria virulence: PrfA master and commander
2011年4月1日 · PrfA, a regulatory protein, integrates a number of environmental cues that signal the transition between these two contrasting lifestyles, activating a set of key virulence factors …