pSC101 ori is a low-copy replication origin that requires the Rep101 protein, which is a temperature-sensitive protein. When bacteria containing a plasmid with this ori is cultured at 37℃, the plasmid will be lost. 1. 发货形式：质粒干粉 (常温运输，存于-20度，请务必先转化提质粒后使 …

Low copy number plasmid from Salmonella. pSC101 was the first plasmid vector used for cloning. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from different BsrDI sites may not be compatible. Efficient cleavage requires at least two copies of the RsrII recognition sequence.

pSC101 is a DNA plasmid that is used as a cloning vector in genetic cloning experiments. pSC101 was the first cloning vector, used in 1973 by Herbert Boyer and Stanley Norman Cohen. Using this plasmid they have demonstrated that a gene from a frog could be transferred into bacterial cells and then expressed by the bacterial cells.

2023年8月29日 · PSC101质粒 分析. Rep101（Ts）蛋白为温度敏感型. A temperature-sensitive replicationsystem restricts the replication of a plasmid that contains the genes of interest at the defined temprerature . Psc101质粒在37℃停止复制，而在30℃才可正常复制。

Plasmid pSC101-AmpR EV from Dr. Tanel Tenson's lab is published in Sci Rep. 2024 Mar 22;14(1):6883. doi: 10.1038/s41598-024-57597-8. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format

在野生型pSC101中，质粒拷贝数PCN（plasmid copy number）由repA的平衡控制，一方面，单体形式的RepA与Ori位点结合并促进质粒复制启动，从而上调PCN，另一方面，RepA在较高的表达水平下，除了二聚化还充当抑制其自身表达的转录抑制因子，从而下调PCN。

pSC101-Donor载体价格质粒购买价格是1200元，pSC101-Donor载体质粒图谱(Vector map)，pSC101-Donor (addgene 140630)载体序列(Sequence)见下文，载体质粒抗性为卡那霉素，质粒大小为4133 bp。

Plasmid pSC101-Donor from Dr. Sheng Yang's lab contains the insert LE, RE, KanR, temperature sensitive pSC101 origin of replication and is published in ACS Synth Biol. 2020 Jun 17. doi: 10.1021/acssynbio.0c00073. This plasmid is available through Addgene.

确定了pSC101自主复制和分配所必需区域的核苷酸序列，pSC101是一种质粒，其复制依赖于大肠杆菌dnaA基因产物。 基本复制区包含一个长编码序列，即rep101，用于由316个氨基酸组成的蛋白质，并且将大小约为37 X 10（3）Mr的多肽鉴定为rep101基因产物。

Let’s consider pSC101. This plasmid is stringently controlled and has a low copy number. Like the E. coli chromosome, it has a single ori and requires the host proteins DnaA and IHF to initiate replication. But pSC101 requires a third component: RepA, a protein encoded by pSC101 itself.