The RecA protein of Escherichia coli is the prototypic DNA strand exchange protein. It assembles on single-stranded DNA to form a helical nucleoprotein filament that is the active species for all RecA protein-dependent functions.

2001年7月24日 · First, RecA binding sites of low and high affinity were identified by using triplet repeats in the presence of ATPγS, a nonhydrolyzable analog of ATP. We find that RecA binds well to sequences rich in pyrimidines and that ssDNA rigidity of stacked purine sequences and secondary structure caused by self-hybridization all hinder polymerization.

2005年2月1日 · The method for specific DNA cleavage using RecA was developed in two laboratories (reviewed recently in Refs 36, 37) and now is known by two names: RARE and RecA-AC. According to the suggested procedure, the presynaptic complex of RecA protein and its corresponding oligonucleotide is used to find the homologous target on long dsDNA.

2007年6月15日 · The roles of bacterial RecA in the evolution and transmission of antibiotic resistance genes make it an attractive target for inhibition by small molecules. We report two complementary fluorescence-based ATPase assays that …

2013年8月10日 · We identify a specific hydrogen bond (serine 292 and N7 of the purine ring) responsible for the purine specificity of ɸ12 P4 catalysed NTP hydrolysis reaction and find that an extraordinary insertion of the C-terminal peptide into the central channel of the hexamer explains the tight coupling of ATPase activity and RNA translocation in ɸ8.

RecA is a major component in the SOS response and repairs damaged DNA using homologous recombination (HR). During HR, RecA could be introducing mutations that lead to drug resistance as a consequence of unfaithful repair.

2009年4月1日 · Escherichia coli RecA is the founding member of the RecA protein family. It is essential for the initiation of repair of DNA breaks via homologous recombination, induction of the DNA damage-induced 'SOS' response, and activation of translesion DNA synthesis, as well as development and transmission of antibiotic resistance genes [1, 2].

The Escherichia coli AlkB protein is a 2-oxoglutarate/Fe(II)-dependent demethylase that repairs alkylated single stranded and double stranded DNA. Immunoaffinity chromatography coupled with mass spectrometry identified RecA, a key factor in ...

2001年7月7日 · We find that RecA binds well to sequences rich in pyrimidines and that ssDNA rigidity of stacked purine sequences and secondary structure caused by self-hybridization all hinder polymerization. We find a correlation between the onset concentration for polymerization and the free energy of ssDNA folding: the onset of polymerization on all ...

1992年6月9日 · The structurally related nucleoside triphosphates, adenosine triphosphate (ATP), purine riboside triphosphate (PTP), inosine triphosphate (ITP), and guanosine triphosphate (GTP), are all hydrolyzed by the recA protein with the same turnover number (17.5 min-1). The S0.5 values for these nucleotides …