2004年2月1日 · We have conducted a survey of proline‐rich (PxxP) motifs in the proteomes of human, mouse, yeast, Mycobacteriumtuberculosis and Plasmodiumfalciparum. Our analyses reveal a strikingly high occurrence of these motifs in each organism, suggesting a wide dependence on protein–protein interaction networks in cellular systems.

2010年11月22日 · Our complex structure reveals a unique type of SH3 interaction based on recognition of tandem PxxP motifs in the ligand. Strikingly, the specificity pocket of IRTKS SH3 has evolved to accommodate a polyproline type II helical peptide analogously to docking of the canonical PxxP by the conserved IRTKS SH3 proline-binding pockets.

2023年8月9日 · The central PxxP motif, which consists of a proline (P) followed by any two amino acids (x) and another proline (P), induces a hinge that significantly affects the antimicrobial activity of some AHPs. The PxxP motif is often found in cathelicidin-derived antimicrobial peptides, including BMAP-27 and PMAP-23 (Yang et al. 2019, 2006a).

2024年4月18日 · By combining biochemical methods and structural biology, we show that the C-terminal SH3 domain of PEX13 mediates intramolecular interactions with a proximal FxxxF motif. The SH3 domain also...

2003年4月22日 · In vitro peptide selection studies revealed that the majority of SH3 domains require the conserved consensus motif PxxP for recognition. In individual SH3 domains, however, this core PxxP motif is flanked by different specificity elements.

2012年8月14日 · This bacterial peptide contains a typical PPII-helical PxxP motif to interact with the xP pocket region of IRTKS SH3 as a Class I ligand, but exploits a unique strategy for interacting with the specificity zone.

2001年1月1日 · Human immunodeficiency virus (HIV) and simian immunodeficiency virus Nef proteins contain a conserved motif with the minimal consensus (PxxP) site for Src homology region 3 (SH3)-mediated protein-protein interactions.

The central hinge induced by a PXXP motif drives conformational flexibility of amphipathic α-helical structures, which may be the driving force for PMAP-23 translocation across the lipid bilayer.

2021年9月9日 · PMAP-23, a cathelicidin-derived host defense peptide, does not cause severe membrane permeabilization, but exerts strong and broad-spectrum bactericidal activity. We have previously shown that it forms an amphipathic α-helical structure with a central hinge induced by the PXXP motif, which is implic …

2010年12月14日 · Our complex structure reveals a unique type of SH3 interaction based on recognition of tandem PxxP motifs in the ligand. Strikingly, the specificity pocket of IRTKS SH3 has evolved to accommodate a polyproline type II helical peptide analogously to docking of the canonical PxxP by the conserved IRTKS SH3 proline-binding pockets.