
Q5® High-Fidelity DNA Polymerase - NEB
Q5 ® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.
Q5® 超保真 DNA 聚合酶 | NEB
Q5 ® 超保真 DNA 聚合酶为保真度和优异性能设立了新标准。 Q5 具有无与伦比的扩增保真度(约为 Taq 的 280 倍),错配率极低。 Q5 是一种新型聚合酶,融合了具有持续合成能力强的 Sso7d DNA 结合域,能够提高反应速度、保真度和稳定性。 如果需要使用含尿嘧啶的 DNA 模板进行扩增或者在 PCR 反应中掺入 dUTP,可以了解 Q5U 热启动超保真 DNA 聚合酶(NEB #M0515)。 Want to try this product? Request a sample. Need a custom/large volume order? Contact us.
Q5® High-Fidelity 2X Master Mix - NEB
Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB T m Calculator is highly recommended. Q5-High Fidelity 2X Master Mix formats allow robust amplification of a broad range of targets with a single formulation.
Q5/Phusion PCR - Bennett Lab Wiki - Rice University Campus Wiki
2024年8月22日 · Q5 is popular because it is characterized to be the highest-fidelity PCR polymerase engineered thus far (1), with a fidelity 280-fold that of Taq polymerase. The NEB PCR Fidelity Estimator can be used to estimate the fraction of a PCR product that is mutation-free.
Q5 超保真 PCR 试剂盒 Q5 High-Fidelity PCR Kit|基因有限公司
Q5 超保真 PCR 试剂盒包含 Q5 超保真 2 X Master Mix、无核酸酶污染的水、凝胶上样染料和 Quick-Load 2-Log DNA Ladder。Q5 超保真 DNA 聚合酶延伸速度 6kb/min;扩增长度 ≤ 20kb;高保真度:> 100 X Taq;1 Unit/50ul 反应体系;平末端。
PCR Using Q5® High-Fidelity DNA Polymerase (M0491) | NEB
2013年12月13日 · Mg ++ concentration of 2.0 mM is optimal for most PCR products generated with Q5 High-Fidelity DNA Polymerase. When used at a final concentration of 1X, the Q5 Reaction Buffer provides the optimal Mg ++ concentration. Amplification of some difficult targets, like GC-rich sequences, may be improved by the addition of 1X Q5 High GC Enhancer.
Q5® 超保真 PCR 试剂盒 | NEB
q5® 超保真 dna 聚合酶是一种具有热稳定性的超高保真 dna 聚合酶, 拥有 3´→5´ 核酸外切酶活性,并且融合了具有增强持续合成能力的 sso7d dna 结合域, 能够进行稳定的 dna 扩增。
The LunaScript Multiplex One-Step RT-PCR Kit (NEB #E1555) offers a streamlined protocol for cDNA synthesis and PCR amplification in a single reaction. It features Luna WarmStart RT and Q5 Hot Start
Q5 High Fidelity DNA Polymerase - New England Biolabs France
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (>100 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates.
While other DNA polymerases can have difficulty amplifying high-GC or high-AT amplicons, Q5 DNA Polymerase displays superior performance for a wide range of templates. 4. Shorter PCR protocols Achieve precision without sacrificing speed. Q5's unique design incorporating the SSo7d processivity-enhancing
- 某些结果已被删除