Q5 ® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.

Q5 ® 超保真 DNA 聚合酶为保真度和优异性能设立了新标准。 Q5 具有无与伦比的扩增保真度（约为 Taq 的 280 倍），错配率极低。 Q5 是一种新型聚合酶，融合了具有持续合成能力强的 Sso7d DNA 结合域，能够提高反应速度、保真度和稳定性。 如果需要使用含尿嘧啶的 DNA 模板进行扩增或者在 PCR 反应中掺入 dUTP，可以了解 Q5U 热启动超保真 DNA 聚合酶（NEB #M0515）。 Want to try this product? Request a sample. Need a custom/large volume order? Contact us.

Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB T m Calculator is highly recommended. Q5-High Fidelity 2X Master Mix formats allow robust amplification of a broad range of targets with a single formulation.

2024年8月22日 · Q5 is popular because it is characterized to be the highest-fidelity PCR polymerase engineered thus far (1), with a fidelity 280-fold that of Taq polymerase. The NEB PCR Fidelity Estimator can be used to estimate the fraction of a PCR product that is mutation-free.

Q5 超保真 PCR 试剂盒包含 Q5 超保真 2 X Master Mix、无核酸酶污染的水、凝胶上样染料和 Quick-Load 2-Log DNA Ladder。Q5 超保真 DNA 聚合酶延伸速度 6kb/min；扩增长度 ≤ 20kb；高保真度：> 100 X Taq；1 Unit/50ul 反应体系；平末端。

2013年12月13日 · Mg ++ concentration of 2.0 mM is optimal for most PCR products generated with Q5 High-Fidelity DNA Polymerase. When used at a final concentration of 1X, the Q5 Reaction Buffer provides the optimal Mg ++ concentration. Amplification of some difficult targets, like GC-rich sequences, may be improved by the addition of 1X Q5 High GC Enhancer.

q5® 超保真 dna 聚合酶是一种具有热稳定性的超高保真 dna 聚合酶， 拥有 3´→5´ 核酸外切酶活性，并且融合了具有增强持续合成能力的 sso7d dna 结合域， 能够进行稳定的 dna 扩增。

The LunaScript Multiplex One-Step RT-PCR Kit (NEB #E1555) offers a streamlined protocol for cDNA synthesis and PCR amplification in a single reaction. It features Luna WarmStart RT and Q5 Hot Start

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (>100 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates.

While other DNA polymerases can have difficulty amplifying high-GC or high-AT amplicons, Q5 DNA Polymerase displays superior performance for a wide range of templates. 4. Shorter PCR protocols Achieve precision without sacrificing speed. Q5's unique design incorporating the SSo7d processivity-enhancing