The focus of this minireview is the replication control of the 39.9-kb plasmid R6K and its derivatives. Historically, this plasmid was thought to have a narrow host range but more recent findings indicate that its derivatives can replicate in a variety of …

R6K载体价格3800元，R6K载体质粒图谱 (Vector map)，载体序列 (Sequence)见下文，载体质粒抗性为氨苄、链霉素，质粒大小为38 kb。 R6K载体是质粒。

Here, we survey the current state of knowledge regarding R6K replication and place individual regulatory elements into a proposed homeostatic model with implications for the biological significance of R6K and its multiple origins of replication.

May 1, 2013 · A survey of investigations into the replication control of plasmid R6K is presented. Special emphasis is placed on the functional diversity of π/π and π/DNA interactions. A simple homeostatic model is offered to account for the regulated usage of R6K’s three replication origins. Prospects for the use of the plasmid in antibiotic discovery ...

Nov 1, 2023 · ORI 的数量众多，常见的复制子有ColE1，pMB1，pSC101，R6K和15A，复制起点的不同可能导致质粒拷贝数的不同。 复制起点的控制可分为松弛型（relaxed）和严紧型（stringent）两种。

Replication of the gamma origin of Escherichi coli plasmid R6K requires pi protein, encoded by the R6K pir gene and many host factors, including DnaA protein. Pi has dual roles, activating replication at low levels and inhibiting replication at high levels.

提供 R6K-Box 载体/质粒图谱，全长序列，抗性，大小和元件信息。

常见的复制子有 ColE1， pMB1， pSC101， R6K 和15A，复制起点的不同可能导致质粒拷贝数的不同。 通常，复制起点的控制可分为松弛型（relaxed）和严紧型（stringent）两种。 质粒的拷贝数与正调控与负调控的相对强度相关，也可通过对复制子进行突变而改变。 例如，pMB1的起始复制子可维持细胞中含有大约20个质粒拷贝，而pUC却能在每个细胞中产生多达700个质粒拷贝。 严紧型质粒：当细胞复制一次时，质粒也复制一次，每个细胞中只有1-2个质粒，质粒的复制与 …

Oct 2, 1998 · The π 35.0 protein of plasmid R6K regulates transcription and replication by binding a DNA sequence motif (TGAGR) arranged either asymmetrically into 22 bp direct repeats (DRs) in the γ origin, or symmetrically into inverted half-repeats (IRs) in the operator of its own gene, pir.

The minimal &replicon of plasmid R6K contains an open reading frame for a 151-amino acid protein in addition to the seven 22-base pair direct nucleotide sequence repeats, the structural gene (pir) for the u initiation protein, and the @-origin sequence that have been shown to be required for replication activity. In