2018年4月14日 · Known RDR1 / 2 / 6 -dependent sRNAs include phased and repeat-associated short interfering RNAs, while known RDR1 / 2 / 6 -independent sRNAs are primarily microRNAs (miRNA) and other hairpin-derived sRNAs. In this study we produced and analyzed sRNA-seq libraries from rdr1 / rdr2 / rdr6 triple mutant plants.

2022年6月6日 · 与载体对照或突变的RDR1相比，野生型RDR1能够显著抑制三种白血病细胞系（Jurkat、K562和NALM6）在免疫缺陷小鼠外周血中的增殖，并最终延长异种移植小鼠的寿命。

2010年4月16日 · We found that Nt-RDR1 does not interfere with RDR6-dependent siRNA accumulation but turns out to suppress RDR6-dependent S-PTGS. Our results, together with previously published data, suggest that RDR1 might have a dual role, contributing, on one hand, to salicylic acid–mediated antiviral defense, and suppressing, on the other hand, the RDR6 ...

2024年8月13日 · (a) RDR1 is responsible for the biosynthesis and amplification of virus-siRNA (vasiRNA). (b) RDR2 cooperates with Pol IV and is responsible for the biosynthesis of heterochromatin siRNA (hsiRNA). (c) RDR6 converts Pol II transcripts into dsRNA under the help of SGS3, and is responsible for the biosynthesis of 21-nt or 22-nt siRNA.

2022年5月30日 · Unlike CMV-∆2b, efficient CMV-2aT∆2b infection occurs only in rdr1 rdr6 double mutant plants because it triggers potent amplification of vsiRNAs by both RDR1 and RDR6 pathways 13,19.

2022年5月26日 · RDR1 delivered by AAV significantly reduced tumor size and weight (Figures 7 E and 7F). qRT-PCR and western blot analysis suggested that AAV-delivered OsRDR1 was able to dramatically increase miRNA expression to further target and silence cell-cycle genes such as CDK6, CCNE2, PLK1, and MCM2 in tumor tissues in vivo (Figures 7 G and 7H).

rna polymerase iii：rna聚合酶iii; rna聚合酶ii的结构; 猪流行性腹泻病毒的rna依赖rna聚合酶的多抗制备及应用; a．有rna指导的dna聚合酶; hcvrna依赖的rna聚合酶rdrp活性测定及抑制剂筛选; rna聚合酶识别启动子; 一种rna依赖性rna聚合酶截短体的筛选方法、截短体及应用; 乳酸环丙沙星靶向病毒rna聚合酶活性

2024年9月24日 · The ability of Nicotiana benthemiana to produce RPs is correlated with a natural mutation within the gene encoding RNA-dependent RNA polymerase 1, Rdr1 (Bally et al. 2015, 2018). Similarly, a knockout mutation in the RDR6 gene in N. benthemiana plants resulted in the accumulation of a larger amount of recombinant green fluorescent proteins ...

Known RDR1/2/6-dependent sRNAs include phased and repeat-associated short interfering RNAs, while known RDR1/2/6-independent sRNAs are primarily microRNAs (miRNA) and other hairpin-derived sRNAs. In this study we produced and analyzed sRNA-seq libraries from rdr1/rdr2/rdr6 triple mutant plants.

In Caenorhabditis elegans, fungi, and plants, genes encoding RDRs are required for RNA silencing (2–6). Arabidopsis has six genes that encode potential RDRs, and genetic evidence suggests that RDR1, RDR2, and RDR6 act in different biological processes with RDR6 being the major RDR acting in post-transcriptional gene silencing (PTGS) (3, 4, 6, 7).