2008年2月26日 · Kruse et al. (1989) studied linkage of a RFLP associated with the lactase-phlorizin hydrolase gene (which they symbolized LPH) to multiple genetic markers from chromosome 2 and found linkage to 2 of them. Harvey et al. (1993) described the regional localization of LCT to 2q21 by PCR analysis of somatic cell hybrids and by in situ hybridization ...

A porcine 17kb genomic fragment was used as probe to map the lactase phlorizin hydrolase (LCT) gene to pig chromosome 15q13 by fluorescence in situ hybridization. Further, a three-allele TaqI RFLP was used to add the LCT gene to the proximal end of …

2011年3月28日 · If a person was suffering from gastrointestinal disturbances but was of the genotype −13,910 C/T or −13,910 T/T then lactose intolerance could most likely be eliminated as the cause. In fact, PCR-RFLP genotyping and polymerase chain reaction minisequencing assays have been developed by other labs for this purpose [13, 14].

2020年6月1日 · Lactase persistence (LP; OMIM # 223100) is the ability to digest milk sugar in adulthood (Ingram et al., 2009). Intestinal lactase activity is regulated by cis -acting elements of the lactase gene (LCT; OMIM *603202), which has …

The lactase persistent (LP) or lactase non-persistent (LNP) state in European adults is genetically determined by a single nucleotide polymorphism (SNP) located 13.9 kb...

The earliest attempts to understand microbiome assembly used gel electrophoresis–based community fingerprinting methods, such as terminal-restriction fragment length polymorphism (T-RFLP) analysis or (automated) ribosomal intergenic spacer analysis (RISA and ARISA).

2011年10月9日 · C/T − 13910 cis-acting regulatory variant located ≈ 14 kb upstream of lactase gene (LCT) completely correlates with lactase phenotype in Indian children. The genetic testing for the C/T − 13910 variant may be helpful in the diagnosis of adult-type hypolactasia in …

2019年2月21日 · Primary hypolactasia is the main cause of lactose intolerance in adults. It is strongly associated with the single genetic variant LCT-13910C>T, located upstream of the lactase encoding gene. Consequently, analysis of LCT-13910C>T has been recommended as a direct genetic test for the trait.

deste trabalho foi estudar a diversidade do gene LCT, da região codificadora do gene, da região promotora proximal e da região enhancer população brasileira. Um total de 1297 indivíduos foram analisados.

The authors validate a denaturing high-performance liquid chromatography (dHPLC)-based assay versus conventional genotype sequencing in detecting the C/T(-13910) polymorphism of LCT and evaluate its prevalence in 2 different Italian geographical areas and in colorectal cancer patients.