In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations, or species or to pinpoint the locations of genes within a sequence.

2017年11月9日 · Most RFLP markers are co-dominant (both alleles in heterozygous sample will be detected) and highly locus-specific. An RFLP probe is a labeled DNA sequence that hybridizes with one or more fragments of the digested DNA sample after they were separated by gel electrophoresis, thus revealing a unique blotting pattern characteristic to a specific ...

Two soybean [Glycine max (L.) Merr.] populations, 'Young' x PI 416 937 and PI 97100 x 'Coker 237', were evaluated with restriction fragment length polymorphism (RFLP) markers to identify additional QTLs related to seed protein and oil.

We have constructed a genetic map for soybean and identified associations between genetic markers and quantitative trait loci. One-hundred-fifty restriction fragment length polymorphisms (RFLPs) were used to identify genetic linkages in an F2 segregating population from an interspecific cross (Glyci …

RFLPs can be used to measure recombination rates which can lead to a genetic map with the distance between RFLP loci measured in centiMorgans. On this web page, you can see how RFLPs are produced and then three examples of applying RFLP analysis: paternity, disease status, and genetic mapping.

1992年1月1日 · When a genetic linkage map saturated with marker loci is available, the genome of a species can be systematically scanned for markers cosegregating with a specific trait of interest. Because of their abundance, RFLPs are the first class of genetic markers allowing the construction of highly saturated linkage maps.

Two soybean [Glycine max (L.) Merr.] populations, 'Young' x PI 416937 and PI 97100 x 'Coker 237', were evaluated with restriction fragment length polymor-phism (RFLP) markers to identify additional QTLs related to seed protein and oil. For the Young x PI 416937 population, 120 F 4-derived lines were secored for segregation at 155 RFLP loci.

Plants hypoploid for specific segments of each of the maize (Zea mays L.) chromosomes were generated using 24 different B-A translocations. Plants carrying each of the B-A translocations were crossed as male parents to inbreds, and sibling progeny hypoploid or not hypoploid for specific chromosomal segments were recovered.

The construction of a restriction fragment length polymorphism (RFLP) linkage map is based on the estimation of recombination frequencies between genetic loci and on the determination of the linear order of loci in linkage groups. RFLP loci can be …

Hypervariable RFLP loci of this special class are known by a number of different names including Variable Number Tandem Repeat (VNTR) loci and minisatellites, which is the more commonly used term today. Minisatellites are composed of unit sequences that range from 10 to 40 bp in length and are tandemly repeated from tens to thousands of times.