一文了解 luciferase报告基因系统的应用 - 知乎 - 知乎专栏
该RGA(reporter gene assay)被构建用来评估靶向TIGHT免疫的治疗性抗体的生物活性功能，由两种工程细胞组成，即CHO-CD112-CD3 scFv cell line和Jurkat-NFAT-TIGIT cell line，二者共 …
干货分享 | 生物活性检测系列一：报告基因法 - 知乎
荧光素酶 （Luciferase）报告基因是一种常用的报告基因，以药物作用机制为基础，通过将荧光素酶报告基因与特定靶点的细胞信号通路或生理过程相关的基因连接，并将其转染到细胞内，它 …
使用RGAugury鉴定基因组中的抗性基因 - 简书
2022年9月26日 · 并通过整合五个程序产生的结果来识别 RGA：BLAST、InterProScan、pfam_scan、nCoil 和 Phobius。 四种不同 RGA 类型的带注释候选被导出为纯文件。 以并行 …
Development and validation of a reporter gene assay for …
2021年12月1日 · In this study, we developed a reporter gene assay (RGA) based on SK-N-MC cells stably expressing firefly luciferase driven by cAMP response element (CRE). The key …
Development of a novel reporter gene assay to evaluate antibody ...
2021年11月1日 · The novel RGA for the determination of ADCP bioactivity was established after selection of effector and target cells and optimization of a series of key assay parameters …
Reporter gene assays for screening and identification of novel …
A reporter gene assay (RGA) is used to investigate the activity of synthetic chemicals mimicking the molting hormones (MHs) and juvenile hormones (JHs) of insects, so-called insect growth …
An improved reporter gene assay for evaluating the biological …
2024年8月18日 · A novel reporter gene assay (RGA) based on the GH/insulin-like growth factor (IGF)-1 axis was developed. Active rhGH can induce the expression of luciferase by activating …
Development and validation of a reporter gene assay to ... - PubMed
In this study, we have developed and validated a reporter gene assay (RGA), in which two kinds of cell lines were engineered to measure the bioactivity of anti-CTLA-4 mAbs.
A simple and robust reporter gene assay for measuring the …
2019年12月12日 · In this study, we developed a reporter gene assay (RGA) based on a RAW264.7 cell line stably expressing luciferase reporter under the control of nuclear factor-κB …
Development and validation of a novel luciferase reporter gene assay …
To develop and validate a novel reporter gene assay (RGA) to detect pyrogen, HL60 cells were transfected with an NF-κB-RE plasmid containing the luciferase gene to generate stably …