2018年1月22日 · Here, we show that RIP3 activates the pyruvate dehydrogenase complex (PDC, also known as PDH), the rate-limiting enzyme linking glycolysis to aerobic respiration, by directly phosphorylating the...

2021年5月5日 · To investigate the ubiquitination status of RIP3 during necrosis, L929 cells were induced to undergo necrosis with TZ. Endogenous RIP3 from cultured L929 cells were immunoprecipitated to...

程序性坏死是一种由受体相互作用蛋白3 (receptor interacting protein 3，RIP3)和混合谱系激酶域样蛋白 (mixed lineage kinase domain-like protein，MLKL)介导的细胞死亡形式，有研究指出哺乳动物雷帕霉素靶蛋白通路可能参与程序性坏死的调控，真核细胞翻译起始因子4E结合蛋白1 (eukaryotic translation initiation factor 4E-binding protein 1，4EBP1)-真核起始因子4E (eukaryotic initiation factor 4E，eIF4E)通路是哺乳动物雷帕霉素靶蛋白最重要的下游分子通路之一，然而 …

2017年11月23日 · Receptor-interacting protein kinase 3 (RIP3) is a critical initiator in mediating necroptosis induced by tumor necrosis factor alpha (TNFα) in L929 cells, so...

2023年7月28日 · Methods: TNF-α/SM-164/Z-VAD-FMK (TSZ), a necroptosis inducer, was used to induce necroptosis in murine fibroblastoid cell line L929. Cell necrosis was observed under an optical microscope. Then, TSZ was added to L929 cells with RIP3 and MLKL gene knockout. Propidium iodide (PI) staining was used to observe cell necrosis.

Cells expressing RIP3 are capable of undergoing necrosis in response to the TNF family of cytokines (Cho et al., 2009; He et al., 2009; Zhang et al., 2009). These cells include mouse fibroblast L929 cells (in which TNF-induced necrosis was first observed), cells from the hematopoietic system, and epithelial cells of the gastrointestinal tract.

2022年9月16日 · Here, we verify and describe a detailed protocol for immunofluorescent detection of phospho-RIPK3 in L929 cells and mouse yolk sacs. We also describe in detail the model construction methods, sample preparation steps, and staining procedures for immunohistochemical labeling of RIPK3 activation in mouse …

2020年7月31日 · We characterized mutual antagonism between RIP3–RHIM and M45–RHIM in necroptosis regulation, which was caused by distinct inter-filament interactions in RIP3, M45 amyloids revealed with atomic...

2014年11月20日 · RIP3 kinase-dead D161N mutant induces spontaneous apoptosis independent of compound, whereas D161G, D143N, and K51A mutants, like wild-type, only trigger apoptosis when compound is present. Accordingly, RIP3-K51A mutant mice (Rip3K51A/K51A) are viable and fertile, in stark contrast to the perinatal lethality of Rip3D161N/D161N mice.

Receptor-interacting protein kinase 3 (RIP3) is a critical initiator in mediating necroptosis induced by tumor necrosis factor alpha (TNFα) in L929 cells, so knockdown of RIP3 inhibits TNFα-induced L929 cell necroptosis. However, RIP3 knockdown was shown to switch TNFα-induced necroptosis to apoptosis in L929 cells in other studies.