To understand RNA functions, revealing the structure of RNA is of utmost importance, which requires sample preparation. We review the latest methods to produce and purify a variation of RNA molecules for different purposes with the main focus on structural biology and biophysics.

Learn about the workflow for rna-seq sample preparation including RNA extraction, target enrichment, library construction, and RNA barcoding.

RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing- and microarray-based methods, RNA-Seq provides far higher coverage and greater resolution of the dynamic nature of the transcriptome.

2023年8月1日 · Molecular detection of SARS-CoV-2 RNA has primarily relied on reverse transcription polymerase chain reaction (RT-PCR) and isothermal amplification techniques, such as loop-mediated amplification (LAMP) and recombinase polymerase amplification (RPA), and the incorporation of CRISPR technology with these techniques.

The key to successfully performing RNA-seq experiments is to provide the core facility with total RNA of sufficient quality and quantity to produce a library for sequencing. The quality of the initial samples is by far the single-most important factor in the whole process.

2020年4月19日 · Using microarray technology, RNA sample pooling strategies have been reported to optimize both the cost of data generation as well as the statistical power for differential gene expression (DGE) analysis.

2011年6月6日 · RNA-seq is revolutionizing the way we study transcriptomes. mRNA can be surveyed without prior knowledge of gene transcripts. Alternative splicing of transcript isoforms and the identification of previously unknown exons are being reported.

Our guide is designed to steer you towards the successful and dependable isolation of ready-to-use RNA, catering to a diverse range of downstream experiments, including RNA-seq and cDNA generation. Ensuring the purity of RNA, especially in the case of precious and rare samples, requires meticulous purification and decontamination procedures.

2014年1月1日 · Here, we provide a step-by-step guide to the sample preparation in RNA-seq and an overview of the bioinformatic analysis that is required following the cDNA sequencing. The transcriptome is the complete set of transcripts in a given cell and its quantity.

This protocol explains how to convert total RNA into a library of template molecules suitable for high throughput DNA sequencing for subsequent cluster generation. The first step in the workflow involves purifying the poly-A containing mRNA molecules using poly-T …