CRISPR reagents can be delivered into the cell as DNA, RNA, or pre-formed ribonucleoproteins (RNPs). RNPs offer numerous advantages over other delivery approaches due to their ability to rapidly target genomic sites and quickly degrade thereafter. Here, we review the production steps and delivery methods for Cas9 RNPs.

2021年1月1日 · Direct delivery of CRISPR/Cas9 system as a ribonucleoprotein (RNP) complex consisting of Cas9 protein and single guide RNA (sgRNA) has emerged as a powerful and widespread method for genome editing due to its advantages of transient genome editing and reduced off-target effects.

2021年1月25日 · Here, we explore the editing dynamics and DNA repair profiles after the delivery of Cas9-guide RNA ribonucleoprotein (RNP) with or without the adeno-associated virus serotype 6 (AAV6) as HDR donors in four cell types. We show that editing profiles have distinct differences among cell lines.

Direct delivery of CRISPR/Cas9 system as a ribonucleoprotein (RNP) complex consisting of Cas9 protein and single guide RNA (sgRNA) has emerged as a powerful and widespread method for genome editing due to its advantages of transient genome editing and reduced off-target effects.

2021年4月29日 · Here we demonstrate that purified PE ribonucleoprotein (RNP) complexes can induce somatic and germline-transmissible mutations in zebrafish and efficiently edit the genomes of human cell lines...

2024年10月17日 · 以核糖核蛋白（RNP）复合物形式直接递送基因组编辑器，有可能解决与基于mRNA和病毒递送CRISPR编辑器相关的几个限制。 特别是与mRNA相比，RNP会引起较低水平的TLR激活，并因为其短的细胞内半衰期而产生最小的非目标DNA损伤。 此外，与基于mRNA的递送方法相比，RNP可能提供更高的体内编辑效率。 鉴于此，10月16日，加州大学伯克利分校Jennifer A. Doudna教授（2020年诺贝尔化学奖得主）和Niren Murthy教授团队合作，在Nature …

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2021年2月10日 · Genetic engineering using CRISPR ribonucleoproteins (RNPs) has become an attractive approach with many advantages: DNA/transgene-free editing, minimal off-target effects, and reduced toxicity due to the rapid degradation of RNPs and the ability to titrate their dosage while maintaining high editing efficiency.

In this review, we will address the main advances about the origin of biological systems from a ribonucleoprotein point of view. Such ideas range from the conceptual formulation of what life is to recent data explaining how the symbiotic, chemical interaction between RNAs and proteins were established on early Earth (Box 1). Box 1-.

RNP-mediated CRISPR genome engineering is a promising technique for quickly screening CRISPR components and CRISPR systems, as well as an effective platform for generating transgene-free genetically engineered plants.