Human retinal pigment epithelial (RPE) cells derived from induced pluripotent stem (iPS) cells have immunosuppressive properties. However, RPE cells are also known as immunogenic cells, and they have major histocompatibility complex expression and produce inflammatory proteins, and thus experience immune rejection after transplantation.

2021年6月18日 · Our data demonstrate that RPE cells can respond to intracellular RNA and induce type I IFN responses via the RIG-I (DExD/H-box helicase 58, DDX58) RNA helicase. In contrast, we showed that RPE cells were unable to directly sense and respond to DNA through the cGAS-STING pathway.

2024年9月30日 · Here we show when MHC-II knockout RPE are transplanted into monkey eyes, they show suppressed immunogenicity with no infiltration of inflammatory cells, leading to successful engraftment. Our...

2020年3月30日 · In vitro differentiation of human pluripotent stem cells into functional retinal pigment epithelial (RPE) cells provides a potentially unlimited source for cell based reparative therapy of...

2017年11月11日 · After RPE cell fixation, we determined whether antibodies present in the serum could detect RPE cells using IHC. The iPS-RPE cells (46a RPE cells or 1121A1 RPE cells) were clearly much more strongly stained with the sera from TLHM1 (Figure 6 A) and S3-2 (Figure 6 C) monkeys that had immune attacks after transplantation compared with the ...

2016年12月12日 · In the developing murine eye, melanin synthesis in the retinal pigment epithelium (RPE) coincides with neurogenesis of retinal ganglion cells (RGCs). Disruption of pigmentation in the albino RPE is associated with delayed neurogenesis in the ventrotemporal retina, the source of ipsilateral RGCs, and a reduced ipsilateral RGC projection.

2022年2月8日 · We stably induced RPE-like cells (iRPE) from human fibroblasts (Fibs) by conditional overexpression of both broad plasticity and lineage-specific transcription factors (TFs). iRPE cells displayed critical RPE benchmarks and significant in …

2018年9月25日 · This analysis showed ADIPOR1 mRNA presence throughout the neural retina and the RPE. We then performed IHC on paraffin-embedded sections of human retinas to test for protein expression of...

2012年7月13日 · Our findings indicate that constitutive activation of c-Met induced stronger RPE cell migration from the laser-induced injury site to outer layer of the retina; similarly, abrogation of c-Met activity in the c-Met fl/fl floxed mice reduced RPE migration into the wounded sites.

An EGFP-tagged BCO2 protein was expressed in human induced pluripotent stem cell-derived retinal pigment epithelium (iPSC-RPE) cells to further investigate BCO2’s subcellular distribution by ICC. Results : No difference was detected between BCO2 protein levels and distribution in the human macula and the peripheral retina, or between sub ...