RSL3 货号T3646，别名RSL3 1S或1S,3R-RSL3，是 谷胱甘肽过氧化物酶4 （GPX4）的抑制剂，可抑制阻断 GSH 合成的半胱氨酸/谷氨酸氨基酸转运系统，是一种 铁死亡（ferroptosis）激活剂。

2023年6月1日 · Small molecules that trigger ferroptosis include RSL3, ML162, and ML210; these compounds are often used in studies of ferroptosis and are generally considered as GPX4 inhibitors. Here, we found that RSL3 and ML162 completely lack capacity of inhibiting the enzymatic activity of recombinant selenoprotein GPX4.

除了抑制RSL3或Erastin诱导的铁死亡外，有研究表明，Fer-1可以提高GSH水平，以抑制细胞的铁死亡。 另一种常用的有效脂质抗氧化剂是脂质抑素-1。 在没有GPX4的情况下，它可以保护细胞免受铁死亡的侵害。

RSL3 ((1S,3R)-RSL3) is an inhibitor of glutathione peroxidase 4 (GPX4) (ferroptosis activator), reduces the expression of GPX4 protein, and induces ferroptotic death of head and neck cancer cell. RSL3 increases the expression of p62 and Nrf2 and inactivates Keap1 in HN3-rslR cells [1] .

2021年9月1日 · RSL3 induces accumulation of ferroptotic PEox species in cardiac mitochondria. Inhibition of DIC and OGC aggravates ferroptosis and increases mtROS, ΔΨ m loss, and mitochondrial GSH depletion. DHLA exerts anti-ferroptotic effects and eliminates PEox in vitro.

丁硫氨酸亚砜胺(bso)可抑制gsh合成、下降gpx4活性，导致铁死亡发生。 RSL3可以直接与GPX4蛋白结合使其失活，诱导脂质ROS产生，导致细胞铁死亡发生。 另外，过表达GPX4细胞表现出对RSL3的抵抗性，抑制其诱导的细胞铁死亡。

2023年8月18日 · Ferroptosis, a form of programed cell death, can be promoted by inhibitors of the xCT transporter (erastin) or GPX4 (RSL3). We found that GPX4, but not the xCT transporter, is selectively...

2023年5月31日 · RSL3 induced ferroptosis by promoting lipid peroxidation, elevating intracellular LIP concentration and ROS level, and blocking GSH-to-GSSH conversion through the inhibition of GPX4 and...

GSH过氧化物酶4抑制剂（RSL3）是一种小分子化合物，用于抑制GSH过氧化物酶4（GPX4）的活性。 RSL3通过与GPX4的活性位点结合，抑制其催化反应，使其失去还原GSSG的功能。 RSL3的作用是抑制GPX4的活性，限制GSSG的还原，从而导致细胞内GSH水平下降，细胞无法有效对抗氧化应激，最终导致细胞死亡。 RSL3具有选择性作用于GPX4的抑制剂，并且在细胞中具有较高的稳定性，能够有效地抑制GPX4的活性。 它被广泛用于研究GPX4的生物学功能，尤其 …

2022年10月25日 · RSL3 treatment inhibited activity of the NRF2-GSH signaling during KLK LUAD cell ferroptosis in vitro and in vivo. The mechanism is that RSL3 was able to directly bind to USP11, a recently identified de-ubiquitinase of NRF2, and inactivate USP11 protein to induce NRF2 protein ubiquitination and degradation in KLK LUAD cells.