In this study, we took into account the most recent circulating RSV variants and designed two quantitative TaqMan one-step RT-PCR assays to detect and quantify both RSV subgroups separately.

This duplex assay targeted a conserved region of the RSV polymerase gene and was validated for analytical sensitivity, specificity, reproducibility and clinical performance with a wide range of respiratory specimens.

2017年2月10日 · We sought to develop a rapid nucleic acid tests for the detection of RSV within minutes, while retaining the high sensitivity achieved with RT-PCR. We developed and evaluated a reverse transcription isothermal nucleic acid amplification method, reverse transcription strand invasion based amplification (RT-SIBA), for the rapid detection of RSV.

2013年6月15日 · We have developed a simple qRT-PCR-based microneutralization assay (qPCR-MN) for RSV amenable to high-throughput applications. Our approach relies upon a commercially available reagent that circumvents sample RNA purification, thereby removing a procedural obstacle that can be labor-intensive and rate-limiting.

2016年9月1日 · In this study, we designed and validated two one-step TaqMan RT-qPCR assays to efficiently detect, quantify and subtype RSV. These assays target the RSV-A and -B N gene and were designed to take into account a large number of …

2021年8月1日 · To enable typing, a simple real-time RT-PCR duplex assay was developed and validated to detect and differentiate human RSV-A and RSV-B. The methodology has been shared with several laboratories to undertake typing …

3 天之前 · EBV, FeLV, RSV, Reo1 and PCV1 were spiked from a range of 1–5 × 10 6 to 1–5 × 10 1 GC/mL. Vertical black arrows indicate the last dilution tested by each participant.

4 天之前 · Additionally, IL-33 was detected in some non-RSV infections. The data obtained supports the feasibility of a combined rapid test detecting simultaneously hRSV and IL-33, which can be useful as a POC test for screening severe cases of hRSV in primary care settings and emergency rooms. ... RT-qPCR presents some disadvantages, which include high ...

6 天之前 · 目前 rsv 感染的诊断方法多样，如经典的病毒空斑试验、组织培养半数感染剂量（tcid 50 ）、实时荧光定量聚合酶链式反应（rt-qpcr）、免疫荧光（if）试验等。 然而，这些方法都存在各自的局限性。

2021年4月1日 · In this study, we demonstrate that defective viral genomes (DVGs) are viral determinants for RSV disease severity and that detection of DVGs at different stages of infection could be used to...