2018年1月24日 · In summary, we propose that mitochondria–lysosome contacts are regulated in two steps: formation and stabilization of contacts promoted by lysosomal GTP-bound RAB7, followed by contact ...

2022年4月4日 · We show that the Rab5-to-Rab7 conversion involves fusion of Rab5-marked early macropinosomes with Rab7-marked late macropinosomes. PripA links the two membrane compartments by interacting with...

Accessory proteins regulate the nucleotide binding state of Rab7: guanine nucleotide exchange factors (GEFs) stimulate exchange of bound GDP for GTP, resulting in Rab7 activation, whereas GTPase activating proteins (GAPs) boost Rab7’s GTP …

2019年8月1日 · Recent evidence shows that in addition to activating and inactivating Rab GTPases, both Rab GEFs and GAPs directly contribute to membrane tethering events during vesicle traffic. Other studies have extended the range of processes, in which Rabs function, and revealed roles for Rabs and their GAPs in the regulation of autophagy.

2018年10月1日 · This review will discuss the GEF and GAPs that control Rab7 nucleotide binding, and thus regulate Rab7’s activity in endolysosomal trafficking and autophagy. It will also consider how bacterial pathogens manipulate Rab7 nucleotide binding to support intracellular invasion and immune evasion.

2011年3月1日 · Like other Rab proteins, Rab7 is activated by a set of upstream regulators that converge on its GEF (guanine-nucleotide exchange factor) and GAP (GTPase activation protein). Rab7 exerts its biological functions through interaction with diverse downstream effectors (Fig. 1).

<p>RabGTPase激活蛋白 (RabGAPs)是一类含有TBC (Tre2/Bub2/Cdc16)结构域的蛋白家族，在Rab活性调节中起着失活Rab的作用。 近年的研究表明，RabGAPs蛋白通过与特定Rab相互作用参与细胞内膜泡运输的调节。...

2010年5月28日 · TBC1D15 functioned as a Rab7 GAP in cells, reducing Rab7 binding to its effector protein RILP, fragmenting the lysosome, and conferring resistance to growth factor withdrawal-induced cell death. In a cellular context, TBC1D15 GAP activity was selective for …

2017年11月20日 · Retromer interacts with the RAB 7‐specific GTP ase‐activating protein (GAP) TBC 1D5. Loss of retromer or TBC 1D5 results in depletion of inactive RAB 7 pools and accumulation of hyperactivated RAB 7‐ GTP on endo‐lysosomes. Hyperactivated RAB 7‐ GTP loses its mobility within the cell.

2010年5月12日 · Active Rab5 and Rab7 localize to early and late endosomes, respectively, where they regulate trafficking of cargo from the plasma membrane to the lysosome (Stenmark, 2009). Rab5 and Rab7 are activated by distinct Rab GEFs.