2022年11月30日 · Here we show in mice that, after oncogenic RAS activation, cancer stem cells rewire their gene expression program and trigger self-propelling, aberrant signalling crosstalk with their tissue...

Here, using an inducible model of human squamous cell carcinoma (SCC), we demonstrate that RAS-dependent suppression of PAR3 (encoded by PARD3) accelerates epithelial disorganization during early tumorigenesis. Diminished PAR3 led to loss of E-cadherin-mediated cell adhesion, which in turn contributed to misoriented cell division.

2020年9月1日 · To determine which RAS-GAPs are concerned with the activation of the RAS/ERK pathway in tongue SCC, we analyzed the relationship between mRNA levels of three RAS-GAPs (RASAL1, NF1, and DAB2IP) and p-ERK1/2 overexpression (Table 1).

2009年8月4日 · To explore the function of Raf in Ras-driven SCC, the authors induced the ablation of Raf-1 in two mouse models: (1) the DMBA/TPA chemical carcinogenesis model in which H-Ras is activated by a codon 61 mutation mutation, and (2) K5-SOS-F transgenic mice, in which Ras activation is achieved via the expression of a constitutively active form of ...

受试者和方法 我们通过 PCR-单链构象多态性 (PCR-SSCP) 分析了 K-RAS、H-RAS 和 N-RAS 基因的突变，以及编码 RAS-GAP 的三个基因（RASAL1、NF1、和 DAB2IP) 通过实时逆转录聚合酶链反应 (RT-PCR) 在 20 例舌鳞状细胞癌中进行。 为了检查 RAS-GAP 和磷酸化 ERK1/2（p-ERK1/2）之间的相互作用是否激活了 RAS 信号通路，我们通过免疫组织化学评估了 p-ERK1/2（一种位于 RAS 下游的激酶）的表达。 最后，在 45 例舌鳞状细胞癌中检查了 p …

2022年11月30日 · Squamous cell carcinomas are triggered by marked elevation of RAS–MAPK signalling and progression from benign papilloma to invasive malignancy1–4. At...

结论 体外沉默Icmt基因可有效抑制CAL-27和SCC-4细胞增殖且诱导凋亡,其作用可能是通过影响K-Ras膜蛋白靶向膜定位,负性调控细胞周期和下调Ras/PI3K/Akt/mTOR信号通路而实现。

Penile Squamous Cell Carcinoma (SCC) is a rare cancer with poor prognosis and limited response to conventional chemotherapy. The genetic and epigenetic alterations of Epidermal Growth Factor Receptor (EGFR)-RAS-RAF signaling in penile SCC are unclear. This study aims to investigate four key members of this pathway in penile SCC.

2016年8月1日 · In this review, we will focus on experimental data gathered from murine models of Ras-initiated SCC where the consequence is known to be activation of this proto-oncogene. Various pathological and molecular analyses have uncovered a …

Objective To explore the prognostic value of combined analysis of squamous cell carci-noma antigen (SCC)and DNA methylation of ras association domain family 1A (RASSF1A)in patients with non-small cell lung cancer (NSCLC).