2020年9月28日 · Here we optimized the GRAB ACh (GPCR-activation-based ACh) sensor to achieve substantially improved sensitivity in ACh detection, as well as reduced downstream coupling to intracellular...

Regulation of neurotransmitter release in the central nervous system is complex. Here, we investigated regulatory mechanisms for acetylcholine (ACh) release from cholinergic neurons by performing superfusion experiments with rat striatal segments after labelling the cellular ACh pool with [³ …

The present data suggest that the hindlimb vasodilator response to ACh in the rat is mediated by two mechanisms with an initial ChTX- and apamin-sensitive, L-NAME-resistant phase not mediated by cytochrome P-450 products and a secondary sustained phase mediated by NO.

2008年2月1日 · The present data suggest that the hindlimb vasodilator response to ACh in the rat is mediated by two mechanisms with an initial ChTX- and apamin-sensitive, l -NAME-resistant phase not mediated by cytochrome P -450 products and a …

Endothelium-dependent vasorelaxation (EDV) in response to acetylcholine (ACh) was measured in aortic rings before and after exposure to pharmacological inhibitors. Relaxation responses to sodium nitroprusside were assessed in endothelium-denuded rings.

2023年6月11日 · Our results show that rats transfected with the AAV-VAChT virus in the MS/VDB region or given DON administration both significantly increased ACh levels in the hippocampus, indicating that both interventions enhanced hippocampal cholinergic properties.

2013年1月31日 · In this study, we investigated cholinergic effects on gain control in rat V1 by combining extracellular single-unit recordings and local microiontophoretic administration of ACh, finding that...

2014年1月1日 · Using immunocytochemistry with the monoclonal antibody against whole rat choline acetyltransferase produced by Cozzari et al. (1990), Parent and Descarries (2008) have characterized the ultrastructural features of thalamic ACh axon terminals in the dorsal lateral geniculate (DLG), parafascicular (PF), and reticular thalamic (Rt) nuclei of adult ...

2018年11月12日 · Rats in the DM group received an intraperitoneal injection of 65 mg/kg streptozotocin and measured blood glucose level after 14 days to confirm DM. Bladder smooth muscle contraction was induced using acetylcholine (ACh, 10 −4 M).

A mechanism by which acetylcholine (ACh) may elicit vasoconstrictor response in coronary vessels was studied in rat hearts perfused at a constant flow rate. In spontaneously beating hearts, bolus injections of ACh and carbachol (CCh) produced biphasic changes in coronary perfusion pressure (CPP): a …