2023年1月31日 · SGS3 interacts with RDR6 and drives it to form siRNA bodies in cytoplasm, which is promoted by SGS3-targeted RNAs. Disrupting SGS3 phase separation abrogates siRNA body assembly and siRNA biogenesis, whereas coexpression of SGS3 and RDR6 induces siRNA body formation in tobacco and yeast cells.

2009年4月17日 · Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cytoplasmic granules.

6 天之前 · SGS3 interacts with CHR11, an ISWI-like chromatin remodeling protein. Whereas AGO1, DCL2, DCL4, and RDR6 have clear functions in RNA cleavage or synthesis, the role of the RNA-binding protein SGS3 ...

2021年8月24日 · We show that RDR6 is recruited to a cleavage fragment by 22-nt siRNA-containing AGO1-RISCs in coordination with SGS3 and SDE5. The SGS3-SDE5-RDR6 multicomponent recognition system and the poly (A) tail inhibition may contribute to securing specificity of siRNA amplification.

These results suggest that RDR6 is specifically recruited to an RNA to which SGS3 or SDE5 binds and that poly(A) tail inhibits dsRNA synthesis by RDR6 even in the copresence of 22-nt siRNA-AGO1, SGS3. and SDE5.

2021年3月1日 · In addition, SUPPRESSOR OF GENE SILENCING 3 (SGS3), the RDR6-interacting protein 7, exhibits phase separation both in vitro and in vivo through its prion-like domains,...

Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cyto-plasmic granules.

The increase in At5g18040 mRNA in SGS3 and SGS2/SDE1/RDR6 mutants is attributable to the absence of sRNA255-like siRNAs in these mutants. These results demonstrate a role for endogenous siRNAs in the regulation of gene expression, and suggest that PTGS plays a central role in the temporal control of shoot development in plants.

We concluded that FVE synchronizes the RDR6/SGS3-DRB4/DCL2/4 channel to promote siRNA production, whereas FVE-8 hijacks dsRNA substrates to prevent the downstream processing. Thus, this study reveals a noncanonical role of the epigenetic factor FVE in PTGS and sheds light on a new regulatory layer in transgene silencing.

2021年6月22日 · Biochemistry assays demonstrate that VISP1 interacted with SGS3 and mediated autophagic degradation of SGS3/RDR6 bodies. Further analyses revealed that overexpression of VISP1, mimicking the sgs3 mutant, impaired biogenesis of endogenous trans‐acting siRNAs and up‐regulated their targets.