The RecF pathway, also called the RecFOR pathway, is a pathway of homologous recombination that repairs DNA in bacteria. It repairs breaks that occur on only one of DNA's two strands, known as single-strand gaps.

2023年4月20日 · In bacteria, one type of homologous-recombination-based DNA-repair pathway involves RecFOR proteins that bind at the junction between single-stranded (ss) and double-stranded (ds) DNA. They...

Here we describe a novel, RecBCD- RecFOR-independent (RecBFI) recombination pathway that is active in ΔrecBCD sbcB15 sbcC (D) ΔrecF (OR) mutants of E. coli. In transductional crosses, these mutants show only four-fold decrease of recombination frequency relative to …

Genetic evidence suggests that the RecF, RecO, and RecR (RecFOR) proteins participate in a common step of DNA recombination and repair, yet the biochemical event requiring collaboration of all three proteins is unknown. Here, we show that the concerted action of the RecFOR complex directs the loadin …

2010年1月15日 · Biochemical studies using RecFOR proteins from E. coli indicate that these proteins act together as mediators of the formation of the pre-synaptic RecA filament onto single stranded DNA. Current models agree on the formation of two complexes, RecFR and RecOR.

2003年5月1日 · Here, we show that the concerted action of the RecFOR complex directs the loading of RecA protein specifically onto gapped DNA that is coated with single-stranded DNA binding (SSB) protein, thereby accelerating DNA strand exchange.

We show that conjugational recombination and DNA repair after UV and gamma irradiation in this mutant are highly dependent on recJ, partially dependent on recFOR, and independent of recQ. These results suggest that the recombination pathway operating in a nuclease-deficient recB recD double mutant is also a hybrid.

2012年10月10日 · Results: RecFOR targets RecA to the 5′-end of DNA gaps, regardless whether terminated by DNA or RNA. Conclusion: RecFOR loads RecA onto RNA-DNA substrates that mimic unfinished lagging strand replication gaps. Significance: This work provides biochemical evidence for recognition by RecF of gaps created when DNA replication halts.

2012年4月1日 · There are at least two pathways in Escherichia coli for homologous recombinational DNA repair: RecBCD and RecFOR. In both cases, these proteins act as mediators for the expression of RecA cellular functions. The RecBCD pathway is involved in most of the conjugational recombination and the repair of double-strand breaks (DSBs) [1], [2], [3].

在细菌中，一种基于同源重组的 DNA 修复途径涉及 RecFOR 蛋白，该蛋白结合在单链 (ss) 和双链 (ds) DNA 之间的连接处。 它们有助于用 RecA 取代最初覆盖 ssDNA 的 SSB 蛋白，从而介导同源序列的搜索。