2020年11月2日 · In this study, we determined how modifying the dose and route of SNP-7/8a immunization influenced CD8 + T cell responses. Mice were vaccinated with SNP-7/8a containing Reps1, an MC38 murine...

2023年8月3日 · Reps1 and Adpgk neoantigen peptides are widely reported and used in MC38 syngenic model. However, they were not included because both were undetected by sequencing. Cell line authentication by STR profiling confirmed that our cells matched that of …

2019年2月14日 · M1 vaccine induces neoantigen specific immune responses against MC38 cells. Mice were vaccinated with three biweekly injections of M1 vaccine, which encodes 10 neoantigens from the MC38 colon cancer and a reference CD8 epitope from CEA, immune responses were evaluated in the periphery blood and in the splenocytes by FC.

These data demonstrate that protection from MC38 in the prophylactic setting is dependent on the magnitude of pre-existing Reps1-specific CD8 T cell responses, which is best induced by heterol-ogous prime boost with i.v. SNP and i.v. ChAdOx1.

2020年11月12日 · Using the MC38 model with forced expression of KRAS-G12D, we combined the 27-mers encoding Adpgk and Copg1 neoantigens with the KRAS-G12D 23-mer in lipoplexes, termed Neo-Lpx.

2020年1月13日 · Here, we show that Reps1 LP is particulate in aqueous solution and induces high magnitude CD8 T-cell responses when admixed with a variety of adjuvants (that is, PP-7/8a, CpG or polyICLC),...

We next evaluated the capacity of nanoSTING-vax to enhance CD8 + T cell responses to two established tumor neoantigenic peptides arising from mutations in the proteins Reps1 (AQLANDVVL) and Adpgk (ASMTNMELM) in the MC38 murine colon adenocarcinoma cell line. 59 In these studies, polymersomes were co-loaded with cGAMP and a synthetic long ...

2023年11月15日 · 近日， 天津医科大学医学技术学院&基础医学院尹海芳教授团队 在国际权威期刊 Molecular Therapy 在线发表了题为“Complete remission of tumors in mice with neoantigen-painted exosomes and anti-PD1 therapy”的研究成果 (2023 Nov 1:S1525-0016 (23)00598-1)。 该研究首次报道了基于血清来源外泌体介导肿瘤新生抗原个体化免疫治疗的新策略。 天津医科大学基础医学院博士研究生张杨和青年教师左冰峰为文章的共同第一作者，尹海芳教授为通讯作者。 …

2018年7月23日 · To test whether LADD can elicit neoantigen-specific CD8 + T cells, we constructed an LADD strain expressing MC38 neoepitopes Adpgk and Reps1 (LADD-Neo) (20). Robust Adpgk + CD8 + T cells were detected by tetramer staining in MC38 tumor-bearing mice treated with LADD-Neo (SI Appendix, Fig. S3 A and B).

Reps1 and Adpgk neoantigen peptides are widely reported and used in MC38 syngenic model. However, they were not included because both were undetected by sequencing. Cell line authentication by STR profiling confirmed that our cells matched that of MC38 from Kerafast.