Rotaviruses (RVs) replicate their segmented, double-stranded RNA genomes in tandem with early virion assembly. In this study, we sought to gain insight into the ultrastructure of RV assembly …

We characterize the assembly pathway of group A rotaviruses observed in situ within cryo-preserved infected cells through the use of cryoelectron tomography of cellular lamellae. Our …

Technical parameters of electron microscope staining procedures (type of stain, pH of stain, and time of staining) influence particle integrity for three groups of rotaviruses. Simian rotavirus …

2014年5月29日 · Here we used distinct electron microscopy techniques to determine the structure of the uncleaved particle in two model rotavirus strains. Our results provide a complete …

2019年5月17日 · Transcribing and replicating a double-stranded genome require protein modules to unwind, transcribe/replicate nucleic acid substrates, and release products. Here we present …

In this paper it is been described method of electronic microscopy applied for discovering characteristic morphology of rotaviruses in samples of stools of infected and hospitalized …

2014年9月11日 · Electron microscopy shows images of particles at various stages of engulfment into tightly fitting membrane invaginations, consistent with the interpretation that rotavirus …

2023年4月12日 · We characterize the assembly pathway of group A rotaviruses observed in situ within cryo-preserved infected cells through the use of cryoelectron tomography of cellular …

Nowadays both direct electron microscopy (DEM) and immune electron microscopy (IEM) are used in the detection of ro taviruses (3, 18). We have previously reported two IEM techniques …

Morphological identification was carried out by electron microscopy, which showed virus particles to be mostly circular and occasionally elliptical, with a diameter of 72–74 nm. No capsule was …