Plasmid pDest_pK7WG2_pRPS5A_CTP OleGFP from Dr. Shin-ichi Arimura's lab contains the insert RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2 and is published in Nat Plants. 2021 Jul;7(7):906-913. doi: 10.1038/s41477-021-00954-6.

2022年9月7日 · We used anti-GFP antibodies in RPS5A::ABP1-GFP roots with chemical fixation and in combinations with high-pressure freezing techniques. Both approaches revealed consistent signals that were...

2021年2月12日 · For editing in Arabidopsis, we previously identified the RPS5a promoter element as highly suitable in direct comparisons (Ordon et al., 2019), and it also proved very efficient together with zCas9i (Grützner et al., 2020).

Here, we report efficiency of cytosine base editors (CBEs) in Arabidopsis thaliana by applying the strong endogenous RPS5a promoter to drive the expression of nickase Cas9 and either rAPOBEC1 from rat (BE3) or the PmCDA1 activation-induced cytidine deaminase from sea lamprey (AIDv2).

从生长素应答报告(如pDR5：GFP或P35S：DiI-Venus)的稳态表达水平推测了内源生长素在幼苗根系中的空间分布，这些生长素在靠近根尖的根分生组织静止中心出现明显的最大值。

2023年2月23日 · We also performed the same experiments using the RPS5A»GFP-ATML1 line and found that GFP-ATML1 was induced in the outermost mesophyll cells upon estradiol treatment, regardless of mannitol ...

Ten independent EC1:AMV:BBM transgenic B. napus lines were crossed with an RPS5A:AMV:GFP marker line to facilitate identification of putative haploid seedlings. RPS5A expression in the root tip was used to distinguish diploid seedlings (green fluorescent protein [GFP]-positive) from haploid seedlings (GFP-negative) in the EC1:AMV:BBM × RPS5A ...

In this case, a single RPS5A promoter drives the expression of both the auxin-sensitive DII-VENUS and TagBFP (encoding blue fluorescent protein, which is not degraded and thus provides a reference for normalization), separated by the 2A sequence.

2004年10月1日 · We generated activator lines in which a modified GAL4 protein is expressed under the control of the RPS5A promoter (pRPS5A-GAL4) and target lines carrying PLT1 or PLT2 coding sequences downstream of the UAS promoter (UAS-PLT).

2021年11月22日 · We introduced the embryonic nuclear envelope marker WOX2 pro:NUP54-GFP (ACE-W11) 22 into the RPS5A-GAL4 background and crossed this line with wild-type or upstream activating sequence (UAS)-bdl to visualize the nuclear volume in RPS5A>>bdl and wild-type control embryos.