以铜绿假单胞菌基因组DNA为模板进行PCR扩增，获得含500 bp启动子和flag序列的RsmA-flag DNA片段，用EcoR I和BamH I对广宿主质粒pLAFR3进行双酶切线性化，RsmA-flag片段与线性化载体pLAFR3在重组酶的作用下连接获得pLAFR3-RsmA-flag表达载体。通过三亲结合实验，将pLAFR3-RsmA-flag ...

RsmA is a posttranscriptional regulatory protein in Pseudomonas aeruginosa that works in tandem with a small non-coding regulatory RNA molecule, RsmB (RsmZ), to regulate the expression of several virulence-related genes, including the N-acyl-homoserine lactone synthase genes lasI and rhlI, and the h …

Basal transcription levels were measured when PAO1 and the rsmA mutant were grown in infection medium in the absence of epithelial cells. Transcript levels were also measured in PAO1 and the rsmA mutant following infection of epithelial cells (inducing conditions).

Posttranscriptional control is known to contribute to the regulation of secondary metabolism and virulence determinants in certain gram-negative bacteria. Here we report the isolation of a Pseudomonas aeruginosa gene which encodes a global translational regulatory protein, RsmA (regulator of secondary metabolites).

2023年2月24日 · Our results elucidate the structural basis of full-length RsmZ binding to RsmA, and provide molecular insights into RsmZ regulation of P. aeruginosa virulence. We first separately purified...

In this study, we demonstrate that RsmA of P. aeruginosa can also be a positive control element, as it is required for swarming motility, rhamnolipid synthesis, and lipase production. Both positive and negative effects of RsmA were found to be antagonized by the small regulatory RNA RsmZ, a homolog of P. fluorescens RsmZ.

为了在全基因组水平上研究潜在调节 rsmA 表达的基因，来自西北大学生命科学学院, 西部资源与现代生物技术省部共建教育部重点实验室的研究人员近期发表文章，以转录后调节基因rsmA 的表达为线索, 对野生型 PAO1(CTX-rsmA)进行转座突变, 得到在 PAO1 全基因组范围 ...

2013年9月3日 · In the Gram-negative pathogen, Pseudomonas aeruginosa, RsmA modulates the expression of ∼9% of the 5,570 predicted open reading frames in the PAO1 genome (Brencic and Lory, 2009; Burrowes et al., 2005). This occurs either directly (by binding their mRNAs and affecting translation rates and stabilities) or indirectly (by regulating the ...

Here we have identified a gene encoding an untranslated regulatory RNA (RsmB), located in the rpoS/ fdxA intergenic region of the P. aeruginosa PAO1 genome. Overexpression of rsmB in P. aeruginosa resulted in an increase in N-acyl-homoserine lactone, pyocyanin and elastase production compared with a marked decrease when rsmA was overexpressed.

Positive control of swarming, rhamnolipid synthesis, and lipase production by the posttranscriptional RsmA/RsmZ system in Pseudomonas aeruginosa PAO1. The global posttranscriptional regulator RsmA modulates production of virulence determinants and N-acylhomoserine lactones in Pseudomonas aeruginosa.